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mmu_ciCibar1_1286

Basic information of circular RNA
PanCircBase ID:

mmu_ciCibar1_1286

mm39_Chr. Position ID:

chr4|12166936|12168222|-

Gene name:

Cibar1

Transcript:

ENSMUST00000108285.9

CircRNA type:

Circular intronic (ci)RNA

Exon/Intron count:

1

Exon/intron indexes:

4

Exon/intron offsets:

0

Genomic length:

1286

Exon/intron sizes in nt:

1286

Flanking exon/introns:

chr4:12165771-12168222

Splice length:

1286

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GTATCCTTTGGATTTTGGTCTTTAAAACAAAAAGGTTTTGTGGGGTATGCAGTACAAAAGTAAATGTGTATGGAAATTATACATCTCTAGTTCTAAGTTGGGAATTTTCCTGTCAGTGGCAAAACGAATCCCATTAGGAATCCTCACTGGAGAAACAGGGCATCTTAGAGCAGCCCCACCTCCTCACCCAGTCTGTTTGCTCCTCCTGACAGACGACCCCCAGTCCATTCAGAAACGTTGATGTCTTTAGGGTAAATTAAAAAGTAAATGCTAATGTCTCAGTGCCCTTACCTGCAGTACTTTTAAGGAAGGGGGGAAATTTTCCTACAATGTCCTTCATTAAAACAAAAACAAACAAAAAAAAAACATGAAATCATAGTGATAACTTTTAGTCAAACCTTCAGGAGATTTCAGTATAATTAAGCTCATAGCAAGGCACATGGTATCTAAATATGAATGTTTGTTATAAAACAGTGCTTCTAGAAAACAATTACTTCCTGAACTAGAATATGTTTCATGCAGTGCTAGCAGCTGTGTACAAAGCACTGACATCTCCCTGCCACTCTTCCCCATTCTAGCTTGCTTGTGTCTCCCTCCTGCCCAAGGTTGCTTCTACCCAGCCAACCTAGCCAGACACCAATAAGGAGAAGAGACTCTTGGGGTATTGGAGGTTGAAAAGCACCTTCATTCCTAAGGGAGTCCCCTGGGCAGTGATTTGGGGCTACACCAATTACCTGGTCTGTCTTTCTTTAGGCCAAAGGTACATATGCCCAACTTTCTATTTTAAAAAAGAAAAAGGGCTAGAGAGATGGCTCATAGGTTAAGAGCACTGACTGCTCTTCCAGACATCCTGAGTTCAATTCCCAGCAACCACATGGTGGCTCACAACCATCTGTAATGGGATCTGATGCCCTTTTCTGATGGGTCTGAAGACAGCTACAGTGTTACTCATGTACATAAAATAAATAGATATAAAAAATAGAACAAAAAGGCAGTTCAGTTGGGCTGGTGCAGAATATGTATTGGACATTTATTGGACATTTATTGGAGCTATGAATTTCAAACCAGCCTGATTTAAGCAAGCTGGACAAACCAGAGAATGCTACAAATTCATTCCTGACAACTAGAGATCTGTCAGACTACAGAGTCACCATTAAGTCAGTAATCAAAATTTGTTTTGTTCTTGGGGTAAAGAGTCTATGAAGGCAGGTAAAATATACCACCTGTAATCCTGCATAGTTGTTGGTTGAATCACAAGTTTCATATTGATCTTTAACATAGAAATG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

GGTAAAGAGTCTATGAAGGCAGGT

59.84

ACTGCATACCCCACAAAACCT

59.85

152

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

GGGTAAAGAGTCTATGAAGGCAGG

60.44

ACTGCATACCCCACAAAACCT

59.85

153

PCR template sequence:

GGGTAAAGAGTCTATGAAGGCAGGTAAAATATACCACCTGTAATCCTGCATAGTTGTTGGTTGAATCACAAGTTTCATATTGATCTTTAACATAGAAATGGTATCCTTTGGATTTTGGTCTTTAAAACAAAAAGGTTTTGTGGGGTATGCAGTACAAAAGTAAATGTGTATGGAAATTATACATCTCTAGTTCTAAGTTG

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

8

Interacting miRNAs (#binding sites)

mmu-miR-141-3p(1), mmu-miR-335-5p(1), mmu-miR-495-3p(1), mmu-miR-29b-1-5p(1), mmu-miR-324-3p(1), mmu-miR-466d-3p(1), mmu-miR-5101(1), mmu-miR-181d-5p(1)

siRNAs for circular RNA silencing
Junction sequence

TAACATAGAAATGGTATCCTTTGGAT

siRNA sets

AAAGGATACCATTTCTATG, AAGGATACCATTTCTATGT

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

NA

NA

NA

NA

NA

ORF sequence:

NA

Peptide sequence:

NA

IRESfinder result

IRESfinder score

0.812198862

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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