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mmu_ciCrebl2_1779
Basic information of circular RNA
PanCircBase ID:
mmu_ciCrebl2_1779
mm39_Chr. Position ID:
chr6|134826263|134828042|+
Gene name:
Crebl2
Transcript:
ENSMUST00000111937.2
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
2
Exon/intron offsets:
0
Genomic length:
1779
Exon/intron sizes in nt:
1779
Flanking exon/introns:
chr6:134826263-134829240
Splice length:
1779
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAAGTCTTTCACCAACAATAAAACACCCGTCCCAACCTGGCAGGTCACGAGGGGCCCAGCAGGACAGGAGCTACAGCTGTCCTTAGCCATCAGTGCTCACATTGGAGACAATGTGGAGCTCTTTTCACACTGGAGGCTTTTTGTTTGCTTTTGATTTTTGAGTAGGGCTTCTCTGTGTAGCCCTGGCTGTCCTAGAATTTACTCTGTAGACCAGGCTGGCCTTGAACTCACAGAGGTCCTCCTGCCTAAGCCTATGCCTAGGATTAAAGGCATGTACCACCACCACCAGATGGGACCAAATACACATTTTAATACAGTGCTGGTGCCAACATTACAGAATGTTTTGATAGGAAAAAAATGGTGACTGGAGAGATGACTCAGCGATTCAGAGTGTTCACTGCTCTTCCAGAGGTCCTGAGTGCAATTCCCAGCAACCACATGGTGGCTCATGACCATCTATGACAGGATCCCGTGCCTTTTTCTGGTATGCAGGTGTACTTGCAAACAAAGTACTCATAAACAAGTAAACCTTAAAAAAAAAAAAAAAGAGCTGGAGTTCTCCCCAAACTGAACAAATAAAACAAAACAAACCCCAAAGATCCCAGTTTTAGAAGGAAAAGGAAAAAGGAAGATGTTAGAGTGGCTCAGCAGACCAAGGTACCTGCCAGCAAGGCTGTTGACCCTCCAGTCCCTAAACCCACCCTTGGCCTGCAGGTGTCCTCTGACCCCCACAGGCATGCCCATGACACTGTAGGAAGTGTAATGAGAAGTTACAGAGGGTGGGGAGGTAGCTCAGCGGGTGGAGTGCTTCTCTAGCATGCACGAGGTCCTGAGTGTGATGCCAGCACTGTATGAACAGTGATGACACATCCTGTGACCAGCACTTAGCAGATGGAGGCAGGAAGAGGAGAAGTTCAGGGTCATTTCAATTGCACAGTGAGTTGGAGGCCAATCTAGGATATATGAGACCCTGTCTTAATCCACCCCTCCCTCCAAATAAACAAAAAGGTGCAGTAGCAGATTTAGGTATAAGCCGTGCTGTATTCATATATGAGCCTACAGGTGACCAGGTGGGGAGTCACAGTGCACATCCATATCTACTGATCTGGAAGGTGCTCACAATGTATCGCTAAGTGGGACAGCTGCACAGCAGCCACAGTTCTGCAAGGAGGCTGCCGTTAAGAGTTTCCATGAGTTTACTCTGCATGATTCATTCAGACTTCATAGGGACTGTAAAAGTGTGAGAATAGATAAAACAATTAAAACCCCTTCACCGTGCACTCAGGGATGAGGTGACAGGTGGGGAAACATGTGGTACTACCATGACTGTTGAAAAGCTGGCTCTTCAAAAAGCAATAAAGCTGGCCATTGTGGTGCATGCCTGGAATCCCTGCACTTGGGGGATGGAGGCAGAAGGATCAGGAGTTCAAGGCCATCCTCTGGACTCTATGAGACTATGTCTGAAAATAAAACTAAAACCCTGCAGGGCTCAGGAGATGACTTAGTGAGCAGCGTACCTTGAGATCAGACCTCAGGACCCACATAGCTGGATATACTGTAGTGCACAATCTGTAATCCCGCTCTCCTTGGCAAGATGGAGGCAGTAAGAGCAGTGTCCGGAAGCTCATGGCTGGCTGCCTGGCATATGCGACTTCTAAGCAAGTGCTATAGCACTGAGCTGCACACAAGCCTGCAGGAGAGCTCTTGTGTCTAGCATTTCCACGTGTGCTGCCTGGACTCTGACTTAGCGTTTTACTGAGTGTATTAAATATTTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGCAGGAGAGCTCTTGTGTC
59.68
CTGTAGCTCCTGTCCTGCTG
59.82
164
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTGCAGGAGAGCTCTTGTGT
59.68
CTGTAGCTCCTGTCCTGCTG
59.82
165
PCR template sequence:
GCTGCACACAAGCCTGCAGGAGAGCTCTTGTGTCTAGCATTTCCACGTGTGCTGCCTGGACTCTGACTTAGCGTTTTACTGAGTGTATTAAATATTTCAGGTAAAGTCTTTCACCAACAATAAAACACCCGTCCCAACCTGGCAGGTCACGAGGGGCCCAGCAGGACAGGAGCTACAGCTGTCCTTAGCCATCAGTGCTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
17
Interacting miRNAs (#binding sites)
mmu-miR-203-3p(2), mmu-miR-124-3p(1), mmu-miR-135a-5p(1), mmu-miR-335-5p(1), mmu-miR-139-5p(1), mmu-miR-351-5p(1), mmu-miR-501-3p(1), mmu-miR-96-5p(1), mmu-miR-370-3p(1), mmu-miR-183-5p(1), mmu-miR-541-5p(1), mmu-miR-207(1), mmu-miR-532-5p(1), mmu-miR-669d-5p(1), mmu-miR-3087-3p(1), mmu-miR-540-3p(1), mmu-miR-670-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TTAAATATTTCAGGTAAAGTCTTTCA
siRNA sets
AAAGACTTTACCTGAAATA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.564182107
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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