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mmu_ciCrtc2_1053

Basic information of circular RNA
PanCircBase ID:

mmu_ciCrtc2_1053

mm39_Chr. Position ID:

chr3|90164718|90165771|+

Gene name:

Crtc2

Transcript:

ENSMUST00000029545.15

CircRNA type:

Circular intronic (ci)RNA

Exon/Intron count:

1

Exon/intron indexes:

3

Exon/intron offsets:

0

Genomic length:

1053

Exon/intron sizes in nt:

1053

Flanking exon/introns:

chr3:90164718-90165771

Splice length:

1053

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

ATATCCTTCAAGAGTGATTTAAGGGCAAAGGAACAGGAAGGGGCTCTTTAGAGACTCACAGAGGGGAGGCGCTTTCTCCTTATATGCTCATGTTCATCTTTCTGCAGTTCTTTTACCCAGTCGTGAAAAGTGACTACAACCTGTGTGTCAGCACAGACTGTTGTGATGGGCAGCTGGTGTTTCTGTGGTTGTGCCCTCCTCAGTGCTGGTCTTGGCTGGCCTTACTTTTGCTCACTTGATTGTAAAGCAGGGCTCCCTGTCACCTGGGCAGGAGATTCAGTCCCTTTTCTCATTCTGTTGTGAACTTTGATTCCTAGAGACGCTACCATGTTTGTGTCCCTTACCCTACTCAGGCATTTGCTTAGGTTGTTCCACCTCTACTATGCTTGGCCTGATTTTTCCCCCTATTTATTTGTTTGTTTATTTGTTTGTTTACTTATTTACTTGACTTTCTGAGCATCTCTCTATGTAGTTCTGGTTGTGCTGGAGCTTGTCCAGGCTGGCCTATGTAGACTAGGTAGACATCCTCCTCCTCTGCTTCCTGAGTGCTAAGATTAAAGAGGTGTGCCAGGCCTGGTTTTCTTTTTTGTTTGTTTGGTTTTGGTTCTGTTTTGAGATGGTTTAATCCTGCAGTCTGGGATTTTTCTGAAACTTGACAGAAAAATGAGGTAGGGTTCAAACTTTTGGTGACGTCCTTGGCACAGCCTCCTAAGTACTGGGATCACAAGTGTAGACCACCATGCCTGCATTTATTCCATTTTATTGTTTGCTTATTTTTGTGGTCCAGGGCCTAACAAATTAGGTGGCCTTCATTGAGATGTAACCCTGGTTTCTCGTCCTTCTTCTTTCCTTCTGTTCTTGTGTGTGTGTGTGTGTGTGTGTCTGTGTGTGTGTGTGTGTGTATGTGTGTGTATGTGTAGAGACACGACATCTCACTATGTTGGGATTACAGCAGTGAGCCACTATATGTGACTTACTTGTATTGCCACACACTGTCTCCCTTATTCTGTCATCTGTTCTCTCTACTTTTTTTCCTTAACTATAGTTATCTAC

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

ATTGCCACACACTGTCTCCC

60.25

CCTCCCCTCTGTGAGTCTCT

59.67

141

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

TATTGCCACACACTGTCTCCC

60

CCTCCCCTCTGTGAGTCTCT

59.67

142

PCR template sequence:

AGTGAGCCACTATATGTGACTTACTTGTATTGCCACACACTGTCTCCCTTATTCTGTCATCTGTTCTCTCTACTTTTTTTCCTTAACTATAGTTATCTACATATCCTTCAAGAGTGATTTAAGGGCAAAGGAACAGGAAGGGGCTCTTTAGAGACTCACAGAGGGGAGGCGCTTTCTCCTTATATGCTCATGTTCATCTT

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

11

Interacting miRNAs (#binding sites)

mmu-miR-26a-5p(1), mmu-miR-298-5p(1), mmu-miR-709(1), mmu-miR-495-3p(1), mmu-miR-434-3p(1), mmu-miR-26b-5p(1), mmu-miR-22-3p(1), mmu-miR-182-5p(1), mmu-miR-466d-3p(1), mmu-miR-224-5p(1), mmu-miR-466b-3p(1)

siRNAs for circular RNA silencing
Junction sequence

TATAGTTATCTACATATCCTTCAAGA

siRNA sets

GAAGGATATGTAGATAACT,AAGGATATGTAGATAACTA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

NA

NA

NA

NA

NA

ORF sequence:

NA

Peptide sequence:

NA

IRESfinder result

IRESfinder score

0.941975011

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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