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mmu_ciDagla_1738

Basic information of circular RNA
PanCircBase ID:

mmu_ciDagla_1738

mm39_Chr. Position ID:

chr19|10240633|10242371|-

Gene name:

Dagla

Transcript:

ENSMUST00000039327.11

CircRNA type:

Circular intronic (ci)RNA

Exon/Intron count:

1

Exon/intron indexes:

6

Exon/intron offsets:

0

Genomic length:

1738

Exon/intron sizes in nt:

1738

Flanking exon/introns:

chr19:10240592-10242371

Splice length:

1738

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GTAAGTACCCATAGTTGCTTGCCTCGGGTCTTCTCTCTGCCATGGAAGTGCCTTTCACTCCTCTGGTAATGCTAGGTGGGTGGGGCTGGTAGGAAGTGGGACATTGTGAGCAGAGTAACAGCCTGCTTGTGGGCCTTTTCGCTGAAAAACTGCCCGACCCTGCAAAGCAGACATTCACCTGTTTCACTGCGGAAACAGAAAGCCTTGATGACTGATTTGGTACCCTTGGAACATGGTTTAATTTAAAAGTACCATGTGGTCAGCAGTGGATGGATCTGGAAGGTCTAGGGTGCTGTTGAGTGCTTAGCCTGGGGCTGGATGGCACACATCTCTGAGGTCGGAGATGTGGATAGACCAATAGTGTTGAGGAGCAAGCTCTGCAGAAGGTGGCTCTGAGCAGGAAGGGAGATAGAACCCCTCAGAGTTGGGTAGGCAGGTTAGCAGCCATCCAGCCCTACCCCTCATTTTGCAGATCAGTCAGCTGAGACCCAGAAAGATGGACTGATTTGCTCAGTATCCCACAGCTAGGCAGTAGCAGAGCTTGAGAAGTCAGAAGACAGTGAAGCGCCCACCTCAGACACCCTGGGGAAGCCTGGAGTGGCCCTTCCCTAACAGTAGCCCCCAGGGACCTTAGAGCGAGTACCTAAGGAAGGAAGGAAGGCCCAGAGTTCCCTGGCAGCTGGGGAGTACCTCATGGGGCTGTCTGGGAATGCCTGAGAGATCCTGGTCCAGGTCTGGCTGCAGGTGTGCCTTGCAGAGGGCAGGGAATGGAGCCCAAGAGGTGGAAACCAGGCTTGCCTGGGCCTTAGGCACACTGTTCACCCCATAAAACAGAGTCAGCACTGCTGTGAGGAGCCAGTGAGAAGAGCACAGTCCAGTTAGACACAGACAGCCAGGGGCAGATCCCAGAGGAGGGATTTTTCTCCAGCTTGTATTTTGTCACCAAAGAACTATACTTGACTGCCAGTCTTTGAAAATTTTACATAACCGCCTGTATGTTCAAACCTGGCGCCCTGAGCTGAGCAGCTGAAGCTGAGCACAGTTCCGTTCCGTGTGGCCCCTTAGAGGTCTGAGCAGGTAGCCACTGGCGTTTTCCTCCTGACTTGCCAGGCACTCAAGGACGAGACCAGTGAGATAGATTCAGTTGCTGTGAGTTTGTTCTCGCTCTGCCCTCTCCTGATAGGTTCAAGGCAACAAAAGGCAGGGGAAACCCAGGGCCACAGCTAGCAGGGCTTGTCTGCCTCTGTTCTTTCCTTTTTACCCAAGGGCTGGGGCCAACTTGAAGCCAGAGAGGCCACCGACTATGAATTGTGCTTCTCACGCACATCACAGGGGCCCCTGAGCTGAGGCTGCTGAGAAGCAAGGGAACCTCGAGGTTTGGGAGATGTAGCAGGACTTCCAGGGTCACAGCTGGTAGGACAGCGGTAGCCGGATGCCTTGTGTGGCTTCACTTCTAGAGAAGGGTTGCCTGGACTTGATCCTGGGTATCTAGAGAGAGAAGAGGTATCCGCCTAGGTTGGCACCTGCGAGCAAAGGGACCTCAGTGGAGAGAAGAGGCCTGGGAGGTGGAGAAGGGTCAGCAGCTAAGATGGTAAAAGAACAGAAACAAAAGAGTCTCCAGGTTCCTGCCATAAGGAGGTGCGGCATGCACGCCTGAGGGCAAAGCTGCCCAGAATTGTAGCGATTTCCGGTCACCTTGCCCTTGGTGTTCACTCATGGTCTCTCCTTTCTCCTGCTG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

GCAAAGCTGCCCAGAATTGT

59.68

CCCACCCACCTAGCATTACC

59.82

163

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

GCAAAGCTGCCCAGAATTGT

59.68

CCCCACCCACCTAGCATTAC

59.82

164

PCR template sequence:

GTGCGGCATGCACGCCTGAGGGCAAAGCTGCCCAGAATTGTAGCGATTTCCGGTCACCTTGCCCTTGGTGTTCACTCATGGTCTCTCCTTTCTCCTGCTGGTAAGTACCCATAGTTGCTTGCCTCGGGTCTTCTCTCTGCCATGGAAGTGCCTTTCACTCCTCTGGTAATGCTAGGTGGGTGGGGCTGGTAGGAAGTGGG

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

23

Interacting miRNAs (#binding sites)

mmu-miR-328-3p(2), mmu-miR-326-3p(2), mmu-miR-210-3p(1), mmu-miR-9-5p(1), mmu-miR-27a-3p(1), mmu-miR-150-5p(1), mmu-miR-222-3p(1), mmu-miR-185-5p(1), mmu-miR-339-5p(1), mmu-miR-381-3p(1), mmu-miR-495-3p(1), mmu-miR-214-3p(1), mmu-miR-434-3p(1), mmu-miR-29b-1-5p(1), mmu-miR-22-3p(1), mmu-miR-127-5p(1), mmu-miR-499-5p(1), mmu-miR-690(1), mmu-miR-29b-2-5p(1), mmu-miR-466a-5p(1), mmu-miR-207(1), mmu-miR-490-3p(1), mmu-miR-296-3p(1)

siRNAs for circular RNA silencing
Junction sequence

CTTTCTCCTGCTGGTAAGTACCCATA

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

NA

NA

NA

NA

NA

ORF sequence:

NA

Peptide sequence:

NA

IRESfinder result

IRESfinder score

0.579782505

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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