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mmu_ciHspa12a_2924

Basic information of circular RNA
PanCircBase ID:

mmu_ciHspa12a_2924

mm39_Chr. Position ID:

chr19|58917397|58920321|-

Gene name:

Hspa12a

Transcript:

ENSMUST00000237297.2

CircRNA type:

Circular intronic (ci)RNA

Exon/Intron count:

1

Exon/intron indexes:

2

Exon/intron offsets:

0

Genomic length:

2924

Exon/intron sizes in nt:

2924

Flanking exon/introns:

chr19:58816770-58920321

Splice length:

2924

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GTAGGACGCGAGCTCACTGTACTGTAAGTACTGGTGTATGCAGAGAGGGACCAGGCACCCTGGGAACAGGCACGCTTACGCTTGTGTACACCAGCGACATCGACTTCACAGCACAGTCAGACCCACGAAGGAAAGTGTGTGTAGAGCATGGACAGCCGTGCTCACTGGCCATAGCTTATAGAGTGGGGCCTGGCGTCTGTCGGCCCTGTTTGGCTCATGCTGTCTCCTAGCTCTGTGCTGGTGGCCCTGGAGACCCAGTCACGTTCGGTACCATATGCAGGCAAGGAGAGATGGCCTCTCTGGTGTTAGGAGCTTGTTTATGCTTTAACATAGTCTGTCTGCTCAGAGGATGGCCTTCTTACCCCTCCTTCCTCATGCCTCCCTCCAATACCAGAGAAAACAAGTGGGCTATTCATGCAAGAAGAGAAAGAGCGAAAGGGGAGGGGAGTACCACCGGCAAACCCCATTGAAAGCCACTGGCTGTTCATTTACATTCACACTTTTTAAAAATGTTAGCTAATGATGTTAACTGGTCCGCCAAAGGTCCTGGCAGGTCAGTTGTAGAAGTGTCATTTCTGCCTAATTAGAGCTCTTGTGGCTGCCCCAGGCCTTCCAGTGCCAGTGGTGACAAGCGGGAGCTGACAGCTTCATTTGCTGCAGGCAGGAAGGGTGAGCCGCTTCTGTTTTCCAACTCAGCCAGGTGTTTGCTGAATGGGGAGGGGCCTGGCCCTATCTGAGCGAGCGAGCATCCGAGATCCTTTTCTTTCACTCTGCCCTCCCAAGTCCTTCCCCTTTGCTTGTCTTGTCTTGTGTCAAGGTCAAGAATGGGCTTTGCTAAATCAGCCCTTTGGATTATCTCTGGGAGGTCGTTAGCAGCCCCAGCCCAGTTGCCAGGGTCCCAGGTCGCAGATGCTGGACGCCCCCTCCCCCCCTTTCCTTAAAAATCAGAGAGAAGGATAAGGGGTCAGGATATTCCGGATAGGAACAATAATGAATAGAAGACAAAACGAAAAACTCTCAGAAAGCTCTACATGGTCGTCTGAGTGTTAAAAATCACTACCAGTAAAATGGCTTAAAGAGAAACAGCTACAGCTCTGCAGACAACGCCATAAGTACCTGAAAAGAGGACTGTGGCCGGAGCTGTTCTTACCCTGTCCCGGGCTGGATTGCACCTCCCACATATTAGGTGTGCCTGCTTGGTTATTTCTTGGTTTGATTTTTCAAGAGCAAAAAAAAATAAAAACAAATGGCAGGCTGAGAGACAAAGAACTGAGGAGGCTAAGAAGCCACTAGTTCTTCATTGTCCCCCACCCGGTTCCAGGTGGCATGTGTTCTGACTTTCTCAGTTGCTTTTCAACTAAACTTGTCAGTTGGTAGGATAATCTTGATTTTTTTTTTTTGTCTTGTTATCATGGTAAAAGAACCTAATGTCATTGGTGCTAGGCACCCCTGACTGCAGCGACTGCTACCCAAGGAGAGGGTCCCCCAGCCCTGACAGGACATCTGGGTGTGAGCCTGACCCATATCCTCTCTAGCAGGTCATGAGTTGAACACAGAAGCACCCGAGGACATATGAACACACATGCACATATGGGGCACACTATTCAGTCTGTGTCACAACAGCAGGATAATACACGGGCACTTGGGAACAGTGCCGTATTACAGGGAGAAACGGAAAATCAATGCTTTATGAAAGGCCAAGACTAAATCCACAGGTCTGAAGAGGTAAGGGGCGTTGGAAGGATAGGGCCTGCACTCCAGAGCTCTACCCCCTTTCCTTGCCTCTCCGTTCCTAGCTGCTCTTTCTGTCTGTTCTCACATGGGACGGTGAGAATTGGGGCCTGGAAAATAGACCATCCATGCCTGGTGTTTGGTTACAGAGGTTAAACAGGATGCCGCACATTAGCACATCTGTGCCACCTTGCCACATACCAGGTGCCCAGTACCAGTGGCTGAACTCTGCCTTCCCAAGTGGTCCATGGCTGCAGAATATGTGTAATTCTCAGCCTCTAGTCAGTGAACCTACTAAGTGGAGATGGAGACTGGCCACTCTGAGCTGTGCTTCTTCAGAGCCTGTCATAGAAGGTCCTTGGGGCATCCTGGAACAAGAAAACTGACAGGATTGGCTCTGCTCAGGAAGGAAACATGGAGAAACCTGGGCTTATTCAAGCAGTTTCCTTTGTTTACCCTGCCCAGGGTTGCCAGTGAAGGGGCTCCTCCATCACTAACTAAAGGTCTTATCCTATGCTGGTTCCTCTCCACCCCACCATGCCACAGTGTATTTCTTTCTCTTCCTCGTAGGTGATCTTTTCCCCGGGCTTCTCATTGTTCTGGGGACTGTTGTTTATTTTCTAGTGCCTTTTCCAGGTGTGTGTGACTTGAACTCCTAAGAACAATTCTAAGCCCTTTCTGGGCTCTGCCTCCTACACCCACCTCCATCTTAGAATCTTGGCTCTTGAAGCCACTACTGCAGTCAGCCTCCTCTGAGGGAGAGAGATTGTTGTGTGGCTCTTGTAAGTGTGAAAAAAACAGCCACTTAAGAGCCTTCTTGTGGATATGAAGACAGGTATTTAAAAATCATGGGGAAAGGATTAGTGGTAGAGAAAACCTTCTATAGCATCCAAGTAAAAATGGAAAAAATAAGCTATTTAGTTACAAAAACTAAAACAAACAAGACACTTGTAAAGATCACAGAGCAAGGATCACGTGGCTGACAGCAGTGGAGTTTGCAGTAACTGGCCACCACAGCGGGGACTCTGGGAAGGTGAAATCATGCTCAGTTAATTTTATTCTCTGAAAGGCAGACCTGACTTGTGTTATTACATTGCTCAGCCCTTGAGCATTTCTTTGAGCAACATTCAGTGTTATATTATGTAAGCTTGACACCAAGAAGAAGGTACTATTGTAGATATTCAATTTAATAA

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

CTCAGCCCTTGAGCATTTCT

57.87

GGTCCCTCTCTGCATACACC

59.53

149

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

CTCAGCCCTTGAGCATTTCT

57.87

CCTGGTCCCTCTCTGCATAC

59.24

152

PCR template sequence:

TTGCTCAGCCCTTGAGCATTTCTTTGAGCAACATTCAGTGTTATATTATGTAAGCTTGACACCAAGAAGAAGGTACTATTGTAGATATTCAATTTAATAAGTAGGACGCGAGCTCACTGTACTGTAAGTACTGGTGTATGCAGAGAGGGACCAGGCACCCTGGGAACAGGCACGCTTACGCTTGTGTACACCAGCGACAT

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

29

Interacting miRNAs (#binding sites)

mmu-miR-335-5p(2), mmu-miR-134-5p(1), mmu-miR-141-3p(1), mmu-miR-135a-5p(1), mmu-miR-101a-3p(1), mmu-miR-29b-3p(1), mmu-miR-29a-3p(1), mmu-miR-29c-3p(1), mmu-miR-101b-3p(1), mmu-miR-132-3p(1), mmu-miR-709(1), mmu-miR-212-3p(1), mmu-miR-370-3p(1), mmu-miR-297a-5p(1), mmu-miR-342-5p(1), mmu-miR-705(1), mmu-miR-27a-5p(1), mmu-miR-680(1), mmu-miR-466a-5p(1), mmu-miR-1187(1), mmu-miR-466p-5p(1), mmu-miR-410-3p(1), mmu-miR-344d-3p(1), mmu-miR-5101(1), mmu-miR-335-3p(1), mmu-miR-484(1), mmu-miR-452-3p(1), mmu-miR-296-3p(1), mmu-miR-338-3p(1)

siRNAs for circular RNA silencing
Junction sequence

TTCAATTTAATAAGTAGGACGCGAGC

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

NA

NA

NA

NA

NA

ORF sequence:

NA

Peptide sequence:

NA

IRESfinder result

IRESfinder score

0.713577262

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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