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mmu_ciMyo18a_321

Basic information of circular RNA
PanCircBase ID:

mmu_ciMyo18a_321

mm39_Chr. Position ID:

chr11|77712012|77712333|+

Gene name:

Myo18a

Transcript:

ENSMUST00000108375.9

CircRNA type:

Circular intronic (ci)RNA

Exon/Intron count:

1

Exon/intron indexes:

10

Exon/intron offsets:

0

Genomic length:

321

Exon/intron sizes in nt:

321

Flanking exon/introns:

chr11:77712012-77713488

Splice length:

321

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GTAATGGGCAGCCTGTCCTGTTACCCAGAATGCCTTGGAGGTCCCTACCTGTTTGTGTTGTTGTTCGTTCTTGTCCCACACGTTAAGTCTTAACATTCGGCTGTCACCAGACAACCTTCCCCCGCCCCCACACATGGTTTGACCTGTACGCTGTCTCAGAACTCTACAACAGAGCAGGCTAGAGAAACATCTCTCTGTCCATGATGGCTTGAGGCTTTCTTGGCCTTAGCTTTCAAGACAGAGTGAGGAGCGAGCGGGTAGGTGGCTTGGATGTAGGGTTGAGGGATGGAAGGTACTCCCCCAGAGTGGCAAGTGACTCTC

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

GGTGGCTTGGATGTAGGGTT

59.67

ACGTGTGGGACAAGAACGAA

59.82

144

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

GGTGGCTTGGATGTAGGGTT

59.67

AACGTGTGGGACAAGAACGA

59.82

145

PCR template sequence:

GGCCTTAGCTTTCAAGACAGAGTGAGGAGCGAGCGGGTAGGTGGCTTGGATGTAGGGTTGAGGGATGGAAGGTACTCCCCCAGAGTGGCAAGTGACTCTCGTAATGGGCAGCCTGTCCTGTTACCCAGAATGCCTTGGAGGTCCCTACCTGTTTGTGTTGTTGTTCGTTCTTGTCCCACACGTTAAGTCTTAACATTCGG

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

4

Interacting miRNAs (#binding sites)

mmu-miR-335-5p(1), mmu-miR-320-3p(1), mmu-miR-499-5p(1), mmu-miR-3470b(1)

siRNAs for circular RNA silencing
Junction sequence

GCAAGTGACTCTCGTAATGGGCAGCC

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIMYO18A_321_ORF_1

0.7272

-0.051526448

0.039712433

129

ORF sequence:

ATGGAAGGTACTCCCCCAGAGTGGCAAGTGACTCTCGTAATGGGCAGCCTGTCCTGTTACCCAGAATGCCTTGGAGGTCCCTACCTGTTTGTGTTGTTGTTCGTTCTTGTCCCACACGTTAAGTCTTAA

Peptide sequence:

MEGTPPEWQVTLVMGSLSCYPECLGGPYLFVLLFVLVPHVKS

IRESfinder result

IRESfinder score

0.696633923

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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