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mmu_ciMyo18a_321
Basic information of circular RNA
PanCircBase ID:
mmu_ciMyo18a_321
mm39_Chr. Position ID:
chr11|77712012|77712333|+
Gene name:
Myo18a
Transcript:
ENSMUST00000108375.9
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
10
Exon/intron offsets:
0
Genomic length:
321
Exon/intron sizes in nt:
321
Flanking exon/introns:
chr11:77712012-77713488
Splice length:
321
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAATGGGCAGCCTGTCCTGTTACCCAGAATGCCTTGGAGGTCCCTACCTGTTTGTGTTGTTGTTCGTTCTTGTCCCACACGTTAAGTCTTAACATTCGGCTGTCACCAGACAACCTTCCCCCGCCCCCACACATGGTTTGACCTGTACGCTGTCTCAGAACTCTACAACAGAGCAGGCTAGAGAAACATCTCTCTGTCCATGATGGCTTGAGGCTTTCTTGGCCTTAGCTTTCAAGACAGAGTGAGGAGCGAGCGGGTAGGTGGCTTGGATGTAGGGTTGAGGGATGGAAGGTACTCCCCCAGAGTGGCAAGTGACTCTC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGTGGCTTGGATGTAGGGTT
59.67
ACGTGTGGGACAAGAACGAA
59.82
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGTGGCTTGGATGTAGGGTT
59.67
AACGTGTGGGACAAGAACGA
59.82
145
PCR template sequence:
GGCCTTAGCTTTCAAGACAGAGTGAGGAGCGAGCGGGTAGGTGGCTTGGATGTAGGGTTGAGGGATGGAAGGTACTCCCCCAGAGTGGCAAGTGACTCTCGTAATGGGCAGCCTGTCCTGTTACCCAGAATGCCTTGGAGGTCCCTACCTGTTTGTGTTGTTGTTCGTTCTTGTCCCACACGTTAAGTCTTAACATTCGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-335-5p(1), mmu-miR-320-3p(1), mmu-miR-499-5p(1), mmu-miR-3470b(1)
siRNAs for circular RNA silencing
Junction sequence
GCAAGTGACTCTCGTAATGGGCAGCC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIMYO18A_321_ORF_1
0.7272
-0.051526448
0.039712433
129
ORF sequence:
ATGGAAGGTACTCCCCCAGAGTGGCAAGTGACTCTCGTAATGGGCAGCCTGTCCTGTTACCCAGAATGCCTTGGAGGTCCCTACCTGTTTGTGTTGTTGTTCGTTCTTGTCCCACACGTTAAGTCTTAA
Peptide sequence:
MEGTPPEWQVTLVMGSLSCYPECLGGPYLFVLLFVLVPHVKS
IRESfinder result
IRESfinder score
0.696633923
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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