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mmu_ciOsbpl8_686

Basic information of circular RNA
PanCircBase ID:

mmu_ciOsbpl8_686

mm39_Chr. Position ID:

chr10|111112419|111113105|+

Gene name:

Osbpl8

Transcript:

ENSMUST00000105275.9

CircRNA type:

Circular intronic (ci)RNA

Exon/Intron count:

1

Exon/intron indexes:

14

Exon/intron offsets:

0

Genomic length:

686

Exon/intron sizes in nt:

686

Flanking exon/introns:

chr10:111112419-111113107

Splice length:

686

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GTATGAGAAGTGTGTGTTACCAAGAGGATCTGTAGGTGCTAGGACATTTTCTGAGAAAGTCTTACTTGGTAAATCACTGTTATTTAGCTTATTGCTAGGTAAGCTTTGATAAGCATTGAACATTCTAGAGCAGGACACTACTGAATACCATGCTGCTGAGCATGGCTGTCGTCACTTGGCTCTTGCGTGGCTCCAGTCAGGCTGCCCACCAGTTTATAATTCTCCTTACATTTGGCTGGGATAGATGATTGCTTTCCATGGTCATCTCATGGCTTTTGAGGAAAGAATTTATTAGTTCCTCGGCTGGTAGTGCTTCCCTACATTGTTAACAGCACAAAAACTGGGTTTTCTGAGCTTTGTGCACCTGCTGTTGGGGTTGGGGGCAGGGCTGGAGGGGCTCCTAAAACCCTCCTCTCTGAAAGCTGTATTATAATCTCAAGTTAGAAATGGTGTATTATCATTTCCACCCAAAATCAAACTTTTTTTTTTTTTTTTTTGTCTTGTGGTACGATTGCCAGGGGGTCCATTTCCTTAGAGACAGAAATCTGGTTACCGTAGTGACTAATACTTATGCTGTTGCTTACCAGCTGTCCTCTTACTCGTGCTGACTTCTGAACGCTTGCTATTAGCACAGTTGCAAGCTTGTTTTCTTAAATGTCCTGACTTTGACTTTTCCTGTGTTCACT

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

GCTGTCCTCTTACTCGTGCT

59.47

AGCACCTACAGATCCTCTTGG

58.89

140

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

GCTGTCCTCTTACTCGTGCT

59.47

TGTCCTAGCACCTACAGATCCT

59.75

146

PCR template sequence:

GCTGTCCTCTTACTCGTGCTGACTTCTGAACGCTTGCTATTAGCACAGTTGCAAGCTTGTTTTCTTAAATGTCCTGACTTTGACTTTTCCTGTGTTCACTGTATGAGAAGTGTGTGTTACCAAGAGGATCTGTAGGTGCTAGGACATTTTCTGAGAAAGTCTTACTTGGTAAATCACTGTTATTTAGCTTATTGCTAGGT

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

9

Interacting miRNAs (#binding sites)

mmu-miR-16-5p(2), mmu-miR-103-3p(1), mmu-miR-150-5p(1), mmu-miR-322-5p(1), mmu-miR-335-5p(1), mmu-miR-214-3p(1), mmu-miR-107-3p(1), mmu-miR-499-5p(1), mmu-miR-369-3p(1)

siRNAs for circular RNA silencing
Junction sequence

TCCTGTGTTCACTGTATGAGAAGTGT

siRNA sets

TCTCATACAGTGAACACAG

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

NA

NA

NA

NA

NA

ORF sequence:

NA

Peptide sequence:

NA

IRESfinder result

IRESfinder score

0.810998772

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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