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mmu_ciPrrc2a_318

Basic information of circular RNA
PanCircBase ID:

mmu_ciPrrc2a_318

mm39_Chr. Position ID:

chr17|35377593|35377911|-

Gene name:

Prrc2a

Transcript:

ENSMUST00000025253.12

CircRNA type:

Circular intronic (ci)RNA

Exon/Intron count:

1

Exon/intron indexes:

12

Exon/intron offsets:

0

Genomic length:

318

Exon/intron sizes in nt:

318

Flanking exon/introns:

chr17:35377559-35377911

Splice length:

318

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GTATGAAGAAAGGGATGGGGCTTACTGGGTGTCAAGTCTGCTAGGCTTAAGGGGATAGTGGGTAGTAAAGATGGGTGTCAGTGGGAGAGAGTGCTAGGTTACGCTATTGAACTGTGAGTGGCGGGGTATGTGGGAGGAGAATCTAAGCATCTTGTAGAACAGAACTTCTGGTTCCTGTTTTGTGTTTTCTGGTGGTGTCACAGGAATACTTGACACTTTAAAGTATTTCTATGGTTCTTAAACAGGATGGCCACTTATTCAGTTCGTATTTTGTTAAATACTTAACTGGGGGTGGAATTTCTGGGATGTTTGACTGTT

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

TGGTTCTTAAACAGGATGGCCA

59.89

TCTCCCACTGACACCCATCT

59.88

175

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

TGGTTCTTAAACAGGATGGCCA

59.89

TCTCTCCCACTGACACCCAT

59.88

177

PCR template sequence:

TAAAGTATTTCTATGGTTCTTAAACAGGATGGCCACTTATTCAGTTCGTATTTTGTTAAATACTTAACTGGGGGTGGAATTTCTGGGATGTTTGACTGTTGTATGAAGAAAGGGATGGGGCTTACTGGGTGTCAAGTCTGCTAGGCTTAAGGGGATAGTGGGTAGTAAAGATGGGTGTCAGTGGGAGAGAGTGCTAGGTT

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

6

Interacting miRNAs (#binding sites)

mmu-miR-26a-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-132-3p(1), mmu-miR-26b-5p(1), mmu-miR-188-3p(1)

siRNAs for circular RNA silencing
Junction sequence

ATGTTTGACTGTTGTATGAAGAAAGG

siRNA sets

TTTCTTCATACAACAGTCA,CTTCATACAACAGTCAAAC,CTTTCTTCATACAACAGTC

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIPRRC2A_318_ORF_1

0.5021

-0.29877965

0.010018009

132

ORF sequence:

ATGGTTCTTAAACAGGATGGCCACTTATTCAGTTCGTATTTTGTTAAATACTTAACTGGGGGTGGAATTTCTGGGATGTTTGACTGTTGTATGAAGAAAGGGATGGGGCTTACTGGGTGTCAAGTCTGCTAG

Peptide sequence:

MVLKQDGHLFSSYFVKYLTGGGISGMFDCCMKKGMGLTGCQVC

IRESfinder result

IRESfinder score

0.775922092

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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