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mmu_ciPrrc2a_318
Basic information of circular RNA
PanCircBase ID:
mmu_ciPrrc2a_318
mm39_Chr. Position ID:
chr17|35377593|35377911|-
Gene name:
Prrc2a
Transcript:
ENSMUST00000025253.12
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
12
Exon/intron offsets:
0
Genomic length:
318
Exon/intron sizes in nt:
318
Flanking exon/introns:
chr17:35377559-35377911
Splice length:
318
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTATGAAGAAAGGGATGGGGCTTACTGGGTGTCAAGTCTGCTAGGCTTAAGGGGATAGTGGGTAGTAAAGATGGGTGTCAGTGGGAGAGAGTGCTAGGTTACGCTATTGAACTGTGAGTGGCGGGGTATGTGGGAGGAGAATCTAAGCATCTTGTAGAACAGAACTTCTGGTTCCTGTTTTGTGTTTTCTGGTGGTGTCACAGGAATACTTGACACTTTAAAGTATTTCTATGGTTCTTAAACAGGATGGCCACTTATTCAGTTCGTATTTTGTTAAATACTTAACTGGGGGTGGAATTTCTGGGATGTTTGACTGTT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGTTCTTAAACAGGATGGCCA
59.89
TCTCCCACTGACACCCATCT
59.88
175
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGTTCTTAAACAGGATGGCCA
59.89
TCTCTCCCACTGACACCCAT
59.88
177
PCR template sequence:
TAAAGTATTTCTATGGTTCTTAAACAGGATGGCCACTTATTCAGTTCGTATTTTGTTAAATACTTAACTGGGGGTGGAATTTCTGGGATGTTTGACTGTTGTATGAAGAAAGGGATGGGGCTTACTGGGTGTCAAGTCTGCTAGGCTTAAGGGGATAGTGGGTAGTAAAGATGGGTGTCAGTGGGAGAGAGTGCTAGGTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-26a-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-132-3p(1), mmu-miR-26b-5p(1), mmu-miR-188-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ATGTTTGACTGTTGTATGAAGAAAGG
siRNA sets
TTTCTTCATACAACAGTCA,CTTCATACAACAGTCAAAC,CTTTCTTCATACAACAGTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIPRRC2A_318_ORF_1
0.5021
-0.29877965
0.010018009
132
ORF sequence:
ATGGTTCTTAAACAGGATGGCCACTTATTCAGTTCGTATTTTGTTAAATACTTAACTGGGGGTGGAATTTCTGGGATGTTTGACTGTTGTATGAAGAAAGGGATGGGGCTTACTGGGTGTCAAGTCTGCTAG
Peptide sequence:
MVLKQDGHLFSSYFVKYLTGGGISGMFDCCMKKGMGLTGCQVC
IRESfinder result
IRESfinder score
0.775922092
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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