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mmu_circAbcc1_390

Basic information of circular RNA
PanCircBase ID:

mmu_circAbcc1_390

mm39_Chr. Position ID:

chr16|14209181|14214340|+

Gene name:

Abcc1

Transcript:

ENSMUST00000130671.8

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

3

Exon/intron indexes:

3,4,5

Exon/intron offsets:

0,2635,5033

Genomic length:

5159

Exon/intron sizes in nt:

126,138,126

Flanking exon/introns:

chr16:14207847-14209181|chr16:14214340-14221853

Splice length:

390

Other database IDs:

circAtlas: mmu-Abcc1_0001

circBase: hsa_circ_0038082, circAtlas: hsa-ABCC1_0001, CIRCpedia: HSA_CIRCpedia_16563

Human Ortholog IDs:
Mature spliced Sequence:

GCCTTAGGATTCTTTCTGTGGATCATCTGCTGGGCAGACCTCTTCTACTCTTTCTGGGAAAGAAGTCAGGGAGTGCTCCGAGCCCCAGTGTTACTGGTCAGCCCAACACTGCTGGGCATCACCATGCTGCTCGCCACCTTTTTGATACAGCTTGAACGGAGGAAGGGAGTCCAATCCTCGGGAATTATGCTTACTTTCTGGCTCGTAGCCCTACTCTGTGCCCTTGCCATCTTGAGATCTAAGATCATCTCTGCCTTAAAAAAGGATGCTCATGTGGACGTGTTTCGAGATTCCACGTTCTATCTGTACTTCACCCTTGTGCTTGTTCAGCTCGTGCTGTCCTGCTTCTCAGACTGCTCACCCCTGTTCTCTGAAACTGTCCATGACCGG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

TTCTCAGACTGCTCACCCCT

60.18

CAGTAACACTGGGGCTCGG

60.08

141

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

CTTCTCAGACTGCTCACCCC

59.75

CAGTAACACTGGGGCTCGG

60.08

142

PCR template sequence:

TTCCACGTTCTATCTGTACTTCACCCTTGTGCTTGTTCAGCTCGTGCTGTCCTGCTTCTCAGACTGCTCACCCCTGTTCTCTGAAACTGTCCATGACCGGGCCTTAGGATTCTTTCTGTGGATCATCTGCTGGGCAGACCTCTTCTACTCTTTCTGGGAAAGAAGTCAGGGAGTGCTCCGAGCCCCAGTGTTACTGGTCA

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

2

Interacting miRNAs (#binding sites)

mmu-miR-103-3p(1), mmu-miR-107-3p(1)

siRNAs for circular RNA silencing
Junction sequence

TGTCCATGACCGGGCCTTAGGATTCT

siRNA sets

AGAATCCTAAGGCCCGGTC

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCABCC1_390_ORF_1

0.9537

0.156397591

0.869069775

657

ORF sequence:

ATGCTGCTCGCCACCTTTTTGATACAGCTTGAACGGAGGAAGGGAGTCCAATCCTCGGGAATTATGCTTACTTTCTGGCTCGTAGCCCTACTCTGTGCCCTTGCCATCTTGAGATCTAAGATCATCTCTGCCTTAAAAAAGGATGCTCATGTGGACGTGTTTCGAGATTCCACGTTCTATCTGTACTTCACCCTTGTGCTTGTTCAGCTCGTGCTGTCCTGCTTCTCAGACTGCTCACCCCTGTTCTCTGAAACTGTCCATGACCGGGCCTTAGGATTCTTTCTGTGGATCATCTGCTGGGCAGACCTCTTCTACTCTTTCTGGGAAAGAAGTCAGGGAGTGCTCCGAGCCCCAGTGTTACTGGTCAGCCCAACACTGCTGGGCATCACCATGCTGCTCGCCACCTTTTTGATACAGCTTGAACGGAGGAAGGGAGTCCAATCCTCGGGAATTATGCTTACTTTCTGGCTCGTAGCCCTACTCTGTGCCCTTGCCATCTTGAGATCTAAGATCATCTCTGCCTTAAAAAAGGATGCTCATGTGGACGTGTTTCGAGATTCCACGTTCTATCTGTACTTCACCCTTGTGCTTGTTCAGCTCGTGCTGTCCTGCTTCTCAGACTGCTCACCCCTGTTCTCTGAAACTGTCCATGACCGG

Peptide sequence:

MLLATFLIQLERRKGVQSSGIMLTFWLVALLCALAILRSKIISALKKDAHVDVFRDSTFYLYFTLVLVQLVLSCFSDCSPLFSETVHDRALGFFLWIICWADLFYSFWERSQGVLRAPVLLVSPTLLGITMLLATFLIQLERRKGVQSSGIMLTFWLVALLCALAILRSKIISALKKDAHVDVFRDSTFYLYFTLVLVQLVLSCFSDCSPLFSETVHDR

IRESfinder result

IRESfinder score

0.832287301

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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