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mmu_circAnkrd27_1056
Basic information of circular RNA
PanCircBase ID:
mmu_circAnkrd27_1056
mm39_Chr. Position ID:
chr7|35311887|35325191|+
Gene name:
Ankrd27
Transcript:
ENSMUST00000040844.16
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
10
Exon/intron indexes:
13,14,15,16,17,18,19,20,21,22
Exon/intron offsets:
0,1992,2565,3385,4384,6852,7895,8080,9842,13184
Genomic length:
13304
Exon/intron sizes in nt:
93,12,88,31,16,93,19,80,00,00,00,000
Flanking exon/introns:
chr7:35307878-35311887|chr7:35325191-35326828
Splice length:
1056
Other database IDs:
circAtlas: mmu-Ankrd27_0016
Human Ortholog IDs:
Mature spliced Sequence:
GACGCTGAGGGATTTGGCGACAGACTATTTCTCAAGCAGCGGATGAACTTGCTGTCCCAGATGACATCAACACCCATCGACTGCCTGTTCAAGCACATCGCCTCTGGTAACCAAAAGGAAGTGGAAAGACTTCTGAGCCAAGATGACCAGGACAAAGACGCCATGCAAAAGATGTGCCACCCACTTTGTTCCTGTGAGGACTGTGAGAAGCTTATCTCTGGGAGGCTGAATGACCCGTCAGTTGTCACTCCTTTCTCCAGAGATGACAGAGGTCAAACACCCCTCCATGTGGCTGCCCTCTGTGGTCAGGCATCCCTCATTGACTTCCTGGTCTCCAAAGGTGCCGTAGTGAACGCCACAGACTATCATGGGTCCACCCCCTTACATCTGGCATGTCAGAAAGGCTTCCAAAGTGTGACGCTGCTGCTGCTGCACTACAAGGCCAGCACTGAGGTTCAGGACAACAACGGCAACACCCCGCTGCACCTGGCCTGCACCTATGGGCAGGAGGATTGCGTGAAGGCCCTGGTCTACTATGATGTCCAGGCCTGCAGATTGGATATTGGTAATGAGAAAGGAGACACAGCCCTGCACATTGCTGCACGCTGGGGTTACGAGGGCATCATAGAGACACTGCTGCAGAATGGAGCCCCCACAGCAGTCCAGAACAGACTGAAGGAAACACCACTCAAGTGTGCTCTGAACTCGAAGATTCTGTCTATCATGGAAGCTCATCACCTATCCTCTGACAGGAGGCCAAGGCCTTCTGAGGTTCCTGCACAGTCCCCTACACGCTCTGTGGATTCCATCAGCCAGGGCTCCTCCACCTCCAGCTTTTCTTCCATATCGGTGAGCTTCAGGCAAGAAGAGGTCAAAAAGGACTACAGGGAGGTAGAAAAGCTTCTAAGGGCAGTTGCTGATGGCGATCTAGAAATGGTACGATACCTTTTGGAGTGGACAGAGGATGACCTAGACGATGTGGAAGACGCCATCAGCACGGTGGATCTCGAATTCTGTCACCCCTTATGCCAGTGTCCCAAGTGTGCTCCAGCTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAGCACGGTGGATCTCGAAT
60.18
TGCTTGAACAGGCAGTCGAT
59.96
159
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CAGCACGGTGGATCTCGAAT
60.18
CGATGTGCTTGAACAGGCAG
59.83
164
PCR template sequence:
GACAGAGGATGACCTAGACGATGTGGAAGACGCCATCAGCACGGTGGATCTCGAATTCTGTCACCCCTTATGCCAGTGTCCCAAGTGTGCTCCAGCTCAGGACGCTGAGGGATTTGGCGACAGACTATTTCTCAAGCAGCGGATGAACTTGCTGTCCCAGATGACATCAACACCCATCGACTGCCTGTTCAAGCACATCG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-103-3p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-320-3p(1), mmu-miR-127-5p(1), mmu-miR-218-5p(1), mmu-miR-107-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TGCTCCAGCTCAGGACGCTGAGGGAT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANKRD27_1056_ORF_1
1.0122
0.304801012
0.999997166
2070
ORF sequence:
ATGAACTTGCTGTCCCAGATGACATCAACACCCATCGACTGCCTGTTCAAGCACATCGCCTCTGGTAACCAAAAGGAAGTGGAAAGACTTCTGAGCCAAGATGACCAGGACAAAGACGCCATGCAAAAGATGTGCCACCCACTTTGTTCCTGTGAGGACTGTGAGAAGCTTATCTCTGGGAGGCTGAATGACCCGTCAGTTGTCACTCCTTTCTCCAGAGATGACAGAGGTCAAACACCCCTCCATGTGGCTGCCCTCTGTGGTCAGGCATCCCTCATTGACTTCCTGGTCTCCAAAGGTGCCGTAGTGAACGCCACAGACTATCATGGGTCCACCCCCTTACATCTGGCATGTCAGAAAGGCTTCCAAAGTGTGACGCTGCTGCTGCTGCACTACAAGGCCAGCACTGAGGTTCAGGACAACAACGGCAACACCCCGCTGCACCTGGCCTGCACCTATGGGCAGGAGGATTGCGTGAAGGCCCTGGTCTACTATGATGTCCAGGCCTGCAGATTGGATATTGGTAATGAGAAAGGAGACACAGCCCTGCACATTGCTGCACGCTGGGGTTACGAGGGCATCATAGAGACACTGCTGCAGAATGGAGCCCCCACAGCAGTCCAGAACAGACTGAAGGAAACACCACTCAAGTGTGCTCTGAACTCGAAGATTCTGTCTATCATGGAAGCTCATCACCTATCCTCTGACAGGAGGCCAAGGCCTTCTGAGGTTCCTGCACAGTCCCCTACACGCTCTGTGGATTCCATCAGCCAGGGCTCCTCCACCTCCAGCTTTTCTTCCATATCGGTGAGCTTCAGGCAAGAAGAGGTCAAAAAGGACTACAGGGAGGTAGAAAAGCTTCTAAGGGCAGTTGCTGATGGCGATCTAGAAATGGTACGATACCTTTTGGAGTGGACAGAGGATGACCTAGACGATGTGGAAGACGCCATCAGCACGGTGGATCTCGAATTCTGTCACCCCTTATGCCAGTGTCCCAAGTGTGCTCCAGCTCAGGACGCTGAGGGATTTGGCGACAGACTATTTCTCAAGCAGCGGATGAACTTGCTGTCCCAGATGACATCAACACCCATCGACTGCCTGTTCAAGCACATCGCCTCTGGTAACCAAAAGGAAGTGGAAAGACTTCTGAGCCAAGATGACCAGGACAAAGACGCCATGCAAAAGATGTGCCACCCACTTTGTTCCTGTGAGGACTGTGAGAAGCTTATCTCTGGGAGGCTGAATGACCCGTCAGTTGTCACTCCTTTCTCCAGAGATGACAGAGGTCAAACACCCCTCCATGTGGCTGCCCTCTGTGGTCAGGCATCCCTCATTGACTTCCTGGTCTCCAAAGGTGCCGTAGTGAACGCCACAGACTATCATGGGTCCACCCCCTTACATCTGGCATGTCAGAAAGGCTTCCAAAGTGTGACGCTGCTGCTGCTGCACTACAAGGCCAGCACTGAGGTTCAGGACAACAACGGCAACACCCCGCTGCACCTGGCCTGCACCTATGGGCAGGAGGATTGCGTGAAGGCCCTGGTCTACTATGATGTCCAGGCCTGCAGATTGGATATTGGTAATGAGAAAGGAGACACAGCCCTGCACATTGCTGCACGCTGGGGTTACGAGGGCATCATAGAGACACTGCTGCAGAATGGAGCCCCCACAGCAGTCCAGAACAGACTGAAGGAAACACCACTCAAGTGTGCTCTGAACTCGAAGATTCTGTCTATCATGGAAGCTCATCACCTATCCTCTGACAGGAGGCCAAGGCCTTCTGAGGTTCCTGCACAGTCCCCTACACGCTCTGTGGATTCCATCAGCCAGGGCTCCTCCACCTCCAGCTTTTCTTCCATATCGGTGAGCTTCAGGCAAGAAGAGGTCAAAAAGGACTACAGGGAGGTAGAAAAGCTTCTAAGGGCAGTTGCTGATGGCGATCTAGAAATGGTACGATACCTTTTGGAGTGGACAGAGGATGACCTAGACGATGTGGAAGACGCCATCAGCACGGTGGATCTCGAATTCTGTCACCCCTTATGCCAGTGTCCCAAGTGTGCTCCAGCTCAG
Peptide sequence:
MNLLSQMTSTPIDCLFKHIASGNQKEVERLLSQDDQDKDAMQKMCHPLCSCEDCEKLISGRLNDPSVVTPFSRDDRGQTPLHVAALCGQASLIDFLVSKGAVVNATDYHGSTPLHLACQKGFQSVTLLLLHYKASTEVQDNNGNTPLHLACTYGQEDCVKALVYYDVQACRLDIGNEKGDTALHIAARWGYEGIIETLLQNGAPTAVQNRLKETPLKCALNSKILSIMEAHHLSSDRRPRPSEVPAQSPTRSVDSISQGSSTSSFSSISVSFRQEEVKKDYREVEKLLRAVADGDLEMVRYLLEWTEDDLDDVEDAISTVDLEFCHPLCQCPKCAPAQDAEGFGDRLFLKQRMNLLSQMTSTPIDCLFKHIASGNQKEVERLLSQDDQDKDAMQKMCHPLCSCEDCEKLISGRLNDPSVVTPFSRDDRGQTPLHVAALCGQASLIDFLVSKGAVVNATDYHGSTPLHLACQKGFQSVTLLLLHYKASTEVQDNNGNTPLHLACTYGQEDCVKALVYYDVQACRLDIGNEKGDTALHIAARWGYEGIIETLLQNGAPTAVQNRLKETPLKCALNSKILSIMEAHHLSSDRRPRPSEVPAQSPTRSVDSISQGSSTSSFSSISVSFRQEEVKKDYREVEKLLRAVADGDLEMVRYLLEWTEDDLDDVEDAISTVDLEFCHPLCQCPKCAPAQ
IRESfinder result
IRESfinder score
0.329410654
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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