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mmu_circArhgap12_184
Basic information of circular RNA
PanCircBase ID:
mmu_circArhgap12_184
mm39_Chr. Position ID:
chr18|6039353|6049358|-
Gene name:
Arhgap12
Transcript:
ENSMUST00000182066.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
8,7
Exon/intron offsets:
0,9923
Genomic length:
10005
Exon/intron sizes in nt:
102,82
Flanking exon/introns:
chr18:6037116-6039353|chr18:6049358-6052861
Splice length:
184
Other database IDs:
CIRCpedia: HSA_CIRCpedia_175919
Human Ortholog IDs:
Mature spliced Sequence:
AAAGAACTGGTTGTCTTCTTGGGCAGTGCTGCAGGGTTCGTCTTTACTTTTTACGAAAACTCAAGGAAGTAGTACAAGCTGGTTTGGGAGTAATCAGTCCAAACCAGAATTCACAGTGGACCTGAAGGGGGCAGTGATTGAAATGGCATCAAAGGACAAATCGAGCAAGAAGAATGTATTTGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTCACAGTGGACCTGAAGGG
59.24
TAAAGACGAACCCTGCAGCA
59.61
121
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCACAGTGGACCTGAAGGGG
61.13
TAAAGACGAACCCTGCAGCA
59.61
120
PCR template sequence:
ATCAGTCCAAACCAGAATTCACAGTGGACCTGAAGGGGGCAGTGATTGAAATGGCATCAAAGGACAAATCGAGCAAGAAGAATGTATTTGAGAAAGAACTGGTTGTCTTCTTGGGCAGTGCTGCAGGGTTCGTCTTTACTTTTTACGAAAACTCAAGGAAGTAGTACAAGCTGGTTTGGGAGTA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-324-3p(2), mmu-miR-150-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GAATGTATTTGAGAAAGAACTGGTTG
siRNA sets
TTCTTTCTCAAATACATTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCARHGAP12_184_ORF_1
0.7758
0.016447673
0.088298232
195
ORF sequence:
ATGTATTTGAGAAAGAACTGGTTGTCTTCTTGGGCAGTGCTGCAGGGTTCGTCTTTACTTTTTACGAAAACTCAAGGAAGTAGTACAAGCTGGTTTGGGAGTAATCAGTCCAAACCAGAATTCACAGTGGACCTGAAGGGGGCAGTGATTGAAATGGCATCAAAGGACAAATCGAGCAAGAAGAATGTATTTGAG
Peptide sequence:
MYLRKNWLSSWAVLQGSSLLFTKTQGSSTSWFGSNQSKPEFTVDLKGAVIEMASKDKSSKKNVFE
IRESfinder result
IRESfinder score
0.732910071
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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