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mmu_circArhgap12_184

Basic information of circular RNA
PanCircBase ID:

mmu_circArhgap12_184

mm39_Chr. Position ID:

chr18|6039353|6049358|-

Gene name:

Arhgap12

Transcript:

ENSMUST00000182066.8

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

2

Exon/intron indexes:

8,7

Exon/intron offsets:

0,9923

Genomic length:

10005

Exon/intron sizes in nt:

102,82

Flanking exon/introns:

chr18:6037116-6039353|chr18:6049358-6052861

Splice length:

184

Other database IDs:

CIRCpedia: HSA_CIRCpedia_175919

Human Ortholog IDs:
Mature spliced Sequence:

AAAGAACTGGTTGTCTTCTTGGGCAGTGCTGCAGGGTTCGTCTTTACTTTTTACGAAAACTCAAGGAAGTAGTACAAGCTGGTTTGGGAGTAATCAGTCCAAACCAGAATTCACAGTGGACCTGAAGGGGGCAGTGATTGAAATGGCATCAAAGGACAAATCGAGCAAGAAGAATGTATTTGAG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

TTCACAGTGGACCTGAAGGG

59.24

TAAAGACGAACCCTGCAGCA

59.61

121

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

TCACAGTGGACCTGAAGGGG

61.13

TAAAGACGAACCCTGCAGCA

59.61

120

PCR template sequence:

ATCAGTCCAAACCAGAATTCACAGTGGACCTGAAGGGGGCAGTGATTGAAATGGCATCAAAGGACAAATCGAGCAAGAAGAATGTATTTGAGAAAGAACTGGTTGTCTTCTTGGGCAGTGCTGCAGGGTTCGTCTTTACTTTTTACGAAAACTCAAGGAAGTAGTACAAGCTGGTTTGGGAGTA

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

2

Interacting miRNAs (#binding sites)

mmu-miR-324-3p(2), mmu-miR-150-5p(1)

siRNAs for circular RNA silencing
Junction sequence

GAATGTATTTGAGAAAGAACTGGTTG

siRNA sets

TTCTTTCTCAAATACATTC

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCARHGAP12_184_ORF_1

0.7758

0.016447673

0.088298232

195

ORF sequence:

ATGTATTTGAGAAAGAACTGGTTGTCTTCTTGGGCAGTGCTGCAGGGTTCGTCTTTACTTTTTACGAAAACTCAAGGAAGTAGTACAAGCTGGTTTGGGAGTAATCAGTCCAAACCAGAATTCACAGTGGACCTGAAGGGGGCAGTGATTGAAATGGCATCAAAGGACAAATCGAGCAAGAAGAATGTATTTGAG

Peptide sequence:

MYLRKNWLSSWAVLQGSSLLFTKTQGSSTSWFGSNQSKPEFTVDLKGAVIEMASKDKSSKKNVFE

IRESfinder result

IRESfinder score

0.732910071

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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