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mmu_circArnt2_846

Basic information of circular RNA
PanCircBase ID:

mmu_circArnt2_846

mm39_Chr. Position ID:

chr7|83954004|84010876|-

Gene name:

Arnt2

Transcript:

ENSMUST00000085077.5

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

7

Exon/intron indexes:

8,7,6,5,4,3,2

Exon/intron offsets:

0,397,6076,38945,42545,49723,56757

Genomic length:

56872

Exon/intron sizes in nt:

8,66,61,03,21,42,14,48,000

Flanking exon/introns:

chr7:83935058-83954004|chr7:84010876-84059062

Splice length:

846

Other database IDs:

circAtlas: mmu-Arnt2_0005

circBase: hsa_circ_0104669, circAtlas: hsa-ARNT2_0004, CIRCpedia: HSA_CIRCpedia_144327

Human Ortholog IDs:
Mature spliced Sequence:

AGATGGCGTCAGACATACCAGGATCTGTGGCCTTGCCTGTTGCCCCCATGGCAGCCACCGGACAGGTGAGAATGGCAGGGGCCATGCCTGCCCGAGGAGGAAAGCGTCGATCCGGAATGGACTTCGATGACGAAGATGGTGAAGGTCCCAGTAAATTCTCAAGAGAGAACCACAGTGAGATTGAGCGGCGCAGGCGGAACAAGATGACTCAATATATTACGGAACTCTCCGACATGGTTCCCACCTGCAGTGCACTGGCTAGGAAGCCAGACAAGCTGACCATCCTGCGCATGGCGGTCTCGCACATGAAGTCCATGAGGGGCACCGGCAACAAATCGACTGACGGCGCCTACAAGCCTTCCTTCCTCACTGAGCAGGAACTGAAGCATCTCATCCTGGAAGCCGCTGATGGATTTCTGTTTGTGGTGGCAGCTGAGACAGGGAGAGTCATCTACGTGTCTGATTCGGTCACTCCTGTCCTGAACCAGCCACAGTCAGAGTGGTTTGGGAGCACGCTTTATGAGCAGGTGCACCCTGATGACGTGGAGAAACTGAGGGAACAGCTGTGCACTTCGGAAAACTCCATGACAGGCCGCATCCTGGACCTGAAGACTGGGACAGTGAAGAAGGAGGGACAGCAGTCATCCATGCGCATGTGTATGGGCTCTCGGCGCTCCTTCATCTGTAGGATGAGGTGTGGAAACGCTCCCTTGGACCACCTGCCTTTGAACAGAATAACCACCATGAGGAAAAGGTTCAGGAATGGCCTTGGCCCTGTGAAAGAAGGAGAAGCCCAGTATGCTGTGGTCCACTGCACAGGTTACATCAAGGCTTGGCCACCAGCAG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

TGGCCCTGTGAAAGAAGGAG

59.6

CCTGCCATTCTCACCTGTCC

60.39

156

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

TGGCCCTGTGAAAGAAGGAG

59.6

CCCTGCCATTCTCACCTGTC

60.39

157

PCR template sequence:

AGGAAAAGGTTCAGGAATGGCCTTGGCCCTGTGAAAGAAGGAGAAGCCCAGTATGCTGTGGTCCACTGCACAGGTTACATCAAGGCTTGGCCACCAGCAGAGATGGCGTCAGACATACCAGGATCTGTGGCCTTGCCTGTTGCCCCCATGGCAGCCACCGGACAGGTGAGAATGGCAGGGGCCATGCCTGCCCGAGGAGG

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

7

Interacting miRNAs (#binding sites)

mmu-miR-152-3p(1), mmu-miR-148b-3p(1), mmu-miR-148a-3p(1), mmu-miR-22-3p(1), mmu-miR-411-3p(1), mmu-miR-207(1), mmu-miR-3105-3p(1)

siRNAs for circular RNA silencing
Junction sequence

TGGCCACCAGCAGAGATGGCGTCAGA

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCARNT2_846_ORF_1

1.0625

0.375855521

0.99997012

1689

ORF sequence:

ATGGCGTCAGACATACCAGGATCTGTGGCCTTGCCTGTTGCCCCCATGGCAGCCACCGGACAGGTGAGAATGGCAGGGGCCATGCCTGCCCGAGGAGGAAAGCGTCGATCCGGAATGGACTTCGATGACGAAGATGGTGAAGGTCCCAGTAAATTCTCAAGAGAGAACCACAGTGAGATTGAGCGGCGCAGGCGGAACAAGATGACTCAATATATTACGGAACTCTCCGACATGGTTCCCACCTGCAGTGCACTGGCTAGGAAGCCAGACAAGCTGACCATCCTGCGCATGGCGGTCTCGCACATGAAGTCCATGAGGGGCACCGGCAACAAATCGACTGACGGCGCCTACAAGCCTTCCTTCCTCACTGAGCAGGAACTGAAGCATCTCATCCTGGAAGCCGCTGATGGATTTCTGTTTGTGGTGGCAGCTGAGACAGGGAGAGTCATCTACGTGTCTGATTCGGTCACTCCTGTCCTGAACCAGCCACAGTCAGAGTGGTTTGGGAGCACGCTTTATGAGCAGGTGCACCCTGATGACGTGGAGAAACTGAGGGAACAGCTGTGCACTTCGGAAAACTCCATGACAGGCCGCATCCTGGACCTGAAGACTGGGACAGTGAAGAAGGAGGGACAGCAGTCATCCATGCGCATGTGTATGGGCTCTCGGCGCTCCTTCATCTGTAGGATGAGGTGTGGAAACGCTCCCTTGGACCACCTGCCTTTGAACAGAATAACCACCATGAGGAAAAGGTTCAGGAATGGCCTTGGCCCTGTGAAAGAAGGAGAAGCCCAGTATGCTGTGGTCCACTGCACAGGTTACATCAAGGCTTGGCCACCAGCAGAGATGGCGTCAGACATACCAGGATCTGTGGCCTTGCCTGTTGCCCCCATGGCAGCCACCGGACAGGTGAGAATGGCAGGGGCCATGCCTGCCCGAGGAGGAAAGCGTCGATCCGGAATGGACTTCGATGACGAAGATGGTGAAGGTCCCAGTAAATTCTCAAGAGAGAACCACAGTGAGATTGAGCGGCGCAGGCGGAACAAGATGACTCAATATATTACGGAACTCTCCGACATGGTTCCCACCTGCAGTGCACTGGCTAGGAAGCCAGACAAGCTGACCATCCTGCGCATGGCGGTCTCGCACATGAAGTCCATGAGGGGCACCGGCAACAAATCGACTGACGGCGCCTACAAGCCTTCCTTCCTCACTGAGCAGGAACTGAAGCATCTCATCCTGGAAGCCGCTGATGGATTTCTGTTTGTGGTGGCAGCTGAGACAGGGAGAGTCATCTACGTGTCTGATTCGGTCACTCCTGTCCTGAACCAGCCACAGTCAGAGTGGTTTGGGAGCACGCTTTATGAGCAGGTGCACCCTGATGACGTGGAGAAACTGAGGGAACAGCTGTGCACTTCGGAAAACTCCATGACAGGCCGCATCCTGGACCTGAAGACTGGGACAGTGAAGAAGGAGGGACAGCAGTCATCCATGCGCATGTGTATGGGCTCTCGGCGCTCCTTCATCTGTAGGATGAGGTGTGGAAACGCTCCCTTGGACCACCTGCCTTTGAACAGAATAACCACCATGAGGAAAAGGTTCAGGAATGGCCTTGGCCCTGTGAAAGAAGGAGAAGCCCAGTATGCTGTGGTCCACTGCACAGGTTACATCAAGGCTTGGCCACCAGCA

Peptide sequence:

MASDIPGSVALPVAPMAATGQVRMAGAMPARGGKRRSGMDFDDEDGEGPSKFSRENHSEIERRRRNKMTQYITELSDMVPTCSALARKPDKLTILRMAVSHMKSMRGTGNKSTDGAYKPSFLTEQELKHLILEAADGFLFVVAAETGRVIYVSDSVTPVLNQPQSEWFGSTLYEQVHPDDVEKLREQLCTSENSMTGRILDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGNAPLDHLPLNRITTMRKRFRNGLGPVKEGEAQYAVVHCTGYIKAWPPAEMASDIPGSVALPVAPMAATGQVRMAGAMPARGGKRRSGMDFDDEDGEGPSKFSRENHSEIERRRRNKMTQYITELSDMVPTCSALARKPDKLTILRMAVSHMKSMRGTGNKSTDGAYKPSFLTEQELKHLILEAADGFLFVVAAETGRVIYVSDSVTPVLNQPQSEWFGSTLYEQVHPDDVEKLREQLCTSENSMTGRILDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGNAPLDHLPLNRITTMRKRFRNGLGPVKEGEAQYAVVHCTGYIKAWPPA

IRESfinder result

IRESfinder score

0.211625594

IRES/non IRES

non-IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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