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mmu_circAsh2l_394
Basic information of circular RNA
PanCircBase ID:
mmu_circAsh2l_394
mm39_Chr. Position ID:
chr8|26324987|26330103|-
Gene name:
Ash2l
Transcript:
ENSMUST00000068892.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
5,4,3,2
Exon/intron offsets:
0,1040,4783,5052
Genomic length:
5116
Exon/intron sizes in nt:
95,89,146,64
Flanking exon/introns:
chr8:26323874-26324987|chr8:26330103-26330609
Splice length:
394
Other database IDs:
circAtlas: hsa-ASH2L_0055
Human Ortholog IDs:
Mature spliced Sequence:
GGATGCAAACTTGGTTGATGTAAGTGGTCTGGAGACAGAATCTTCTAATGGAAAAGATACTTTGGAAGGTACCGGAGACACTTCAGAAGTGATGGATACCCAGGCGGGCTCTGTGGATGAGGAGAATGGGCGGCAGTTGGGAGAAGTGGAGTTGCAGTGTGGGATATGTACAAAATGGTTCACCGCTGACACCTTTGGAATAGACACGTCGTCATGTCTGCCTTTTATGACCAACTACAGCTTCCATTGCAATGTGTGCCATCACAGTGGGAATACCTACTTTCTCCGGAAGCAAGCAAATTTAAAAGAAATGTGCCTTAGTGCCTTGGCGAATTTGACGTGGCAGTCTCGAACACAGGATGAACATCCAAAAACCATGTTCTCCAAAGATAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTTGACGTGGCAGTCTCGAA
59.9
AGTGTCTCCGGTACCTTCCA
59.89
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCTTGGCGAATTTGACGTGG
60.11
AGTGTCTCCGGTACCTTCCA
59.89
153
PCR template sequence:
AGCAAATTTAAAAGAAATGTGCCTTAGTGCCTTGGCGAATTTGACGTGGCAGTCTCGAACACAGGATGAACATCCAAAAACCATGTTCTCCAAAGATAAGGGATGCAAACTTGGTTGATGTAAGTGGTCTGGAGACAGAATCTTCTAATGGAAAAGATACTTTGGAAGGTACCGGAGACACTTCAGAAGTGATGGATACC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-150-5p(1), mmu-miR-207(1), mmu-miR-188-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CTCCAAAGATAAGGGATGCAAACTTG
siRNA sets
AAGTTTGCATCCCTTATCT, TTTGCATCCCTTATCTTTG, TTGCATCCCTTATCTTTGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCASH2L_394_ORF_1
0.7594
0.141504413
0.456364152
429
ORF sequence:
ATGAACATCCAAAAACCATGTTCTCCAAAGATAAGGGATGCAAACTTGGTTGATGTAAGTGGTCTGGAGACAGAATCTTCTAATGGAAAAGATACTTTGGAAGGTACCGGAGACACTTCAGAAGTGATGGATACCCAGGCGGGCTCTGTGGATGAGGAGAATGGGCGGCAGTTGGGAGAAGTGGAGTTGCAGTGTGGGATATGTACAAAATGGTTCACCGCTGACACCTTTGGAATAGACACGTCGTCATGTCTGCCTTTTATGACCAACTACAGCTTCCATTGCAATGTGTGCCATCACAGTGGGAATACCTACTTTCTCCGGAAGCAAGCAAATTTAAAAGAAATGTGCCTTAGTGCCTTGGCGAATTTGACGTGGCAGTCTCGAACACAGGATGAACATCCAAAAACCATGTTCTCCAAAGATAAG
Peptide sequence:
MNIQKPCSPKIRDANLVDVSGLETESSNGKDTLEGTGDTSEVMDTQAGSVDEENGRQLGEVELQCGICTKWFTADTFGIDTSSCLPFMTNYSFHCNVCHHSGNTYFLRKQANLKEMCLSALANLTWQSRTQDEHPKTMFSKDK
IRESfinder result
IRESfinder score
0.435572913
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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