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mmu_circAtp6ap2_821

Basic information of circular RNA
PanCircBase ID:

mmu_circAtp6ap2_821

mm39_Chr. Position ID:

chrX|12460999|12475193|+

Gene name:

Atp6ap2

Transcript:

ENSMUST00000033313.3

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

7

Exon/intron indexes:

2,3,4,5,6,7,8

Exon/intron offsets:

0,1395,10106,10357,11520,12170,14074

Genomic length:

14194

Exon/intron sizes in nt:

13,11,32,96,13,85,41,50,000

Flanking exon/introns:

chrX:12454174-12460999|chrX:12475193-12481920

Splice length:

821

Other database IDs:

circAtlas: mmu-Atp6ap2_0002

circAtlas: hsa-ATP6AP2_0001, CIRCpedia: HSA_CIRCpedia_65784

Human Ortholog IDs:
Mature spliced Sequence:

GTGCTTTAGGGAATGAATTTAGCATATTAAGATCGCCAGGGTCTGTTGTTTTCCGAAATGGAAATTGGCCTATACCAGGAGATCGAATCCCAGACGTAGCTGCACTGTCCATGGGCTTCTCTGTAAAGGAAGACCTGTCTTGGCCAGGGCTTGCCGTGGGTAACCTATTCCACCGGCCACGGGCTACCATTATGGTGATGGTGAAGGGGGTGGATAAACTGGCACTTCCGGCAGGCAGCGTCATCTCCTACCCTTTGGAGAATGCAGTTCCTTTTAGTCTAGACAGCGTTGCAAATTCCATTCACTCCTTATTTTCGGAGGAAACACCTGTAGTTTTGCAGTTGGCTCCCAGCGAGGAGAGAGTGTATATGGTGGGGAAAGCAAACTCGGTGTTTGAAGACCTTTCAGTCACATTGCGGCAGCTCCGTAACCGCCTGTTTCAAGAAAACTCCCTTCTCAACTCTCTTCCCCTCAATTCTCTGAGTAGGAATAATGAGGTTGACCTGCTCTTTCTCTCCGAACTGCAAGTGCTACATGATATTTCCAGTTTGTTGTCTCGTCATAAGCATCTAGCCAAGGACCATTCACCCGACTTGTATTCATTGGAGCTGGCAGGTTTGGATGAACTTGGGAAGCGTTATGGGGAAGACTCTGAACAGTTCAGGGATGCTTCTAAGATCCTTGTTGATGCTCTCCAAAAGTTTGCAGATGACATGTACAGTCTCTATGGTGGGAACGCAGTGGTAGAGTTAGTGACTGTCAAATCATTCGACACATCCCTTGTGAGGAAGTCAAGGACCATCCTTGAGGCAAAACAAGAG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

TCTATGGTGGGAACGCAGTG

59.75

CAACAGACCCTGGCGATCTT

60.04

146

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

TCTATGGTGGGAACGCAGTG

59.75

AAAACAACAGACCCTGGCGA

60.11

150

PCR template sequence:

TCTCTATGGTGGGAACGCAGTGGTAGAGTTAGTGACTGTCAAATCATTCGACACATCCCTTGTGAGGAAGTCAAGGACCATCCTTGAGGCAAAACAAGAGGTGCTTTAGGGAATGAATTTAGCATATTAAGATCGCCAGGGTCTGTTGTTTTCCGAAATGGAAATTGGCCTATACCAGGAGATCGAATCCCAGACGTAGC

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

4

Interacting miRNAs (#binding sites)

mmu-miR-34c-5p(1), mmu-miR-148a-3p(1), mmu-miR-185-5p(1), mmu-miR-670-5p(1)

siRNAs for circular RNA silencing
Junction sequence

GGCAAAACAAGAGGTGCTTTAGGGAA

siRNA sets

TTCCCTAAAGCACCTCTTG

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCATP6AP2_821_ORF_1

1.0441

0.152923216

0.922810624

720

ORF sequence:

ATGGGCTTCTCTGTAAAGGAAGACCTGTCTTGGCCAGGGCTTGCCGTGGGTAACCTATTCCACCGGCCACGGGCTACCATTATGGTGATGGTGAAGGGGGTGGATAAACTGGCACTTCCGGCAGGCAGCGTCATCTCCTACCCTTTGGAGAATGCAGTTCCTTTTAGTCTAGACAGCGTTGCAAATTCCATTCACTCCTTATTTTCGGAGGAAACACCTGTAGTTTTGCAGTTGGCTCCCAGCGAGGAGAGAGTGTATATGGTGGGGAAAGCAAACTCGGTGTTTGAAGACCTTTCAGTCACATTGCGGCAGCTCCGTAACCGCCTGTTTCAAGAAAACTCCCTTCTCAACTCTCTTCCCCTCAATTCTCTGAGTAGGAATAATGAGGTTGACCTGCTCTTTCTCTCCGAACTGCAAGTGCTACATGATATTTCCAGTTTGTTGTCTCGTCATAAGCATCTAGCCAAGGACCATTCACCCGACTTGTATTCATTGGAGCTGGCAGGTTTGGATGAACTTGGGAAGCGTTATGGGGAAGACTCTGAACAGTTCAGGGATGCTTCTAAGATCCTTGTTGATGCTCTCCAAAAGTTTGCAGATGACATGTACAGTCTCTATGGTGGGAACGCAGTGGTAGAGTTAGTGACTGTCAAATCATTCGACACATCCCTTGTGAGGAAGTCAAGGACCATCCTTGAGGCAAAACAAGAGGTGCTTTAG

Peptide sequence:

MGFSVKEDLSWPGLAVGNLFHRPRATIMVMVKGVDKLALPAGSVISYPLENAVPFSLDSVANSIHSLFSEETPVVLQLAPSEERVYMVGKANSVFEDLSVTLRQLRNRLFQENSLLNSLPLNSLSRNNEVDLLFLSELQVLHDISSLLSRHKHLAKDHSPDLYSLELAGLDELGKRYGEDSEQFRDASKILVDALQKFADDMYSLYGGNAVVELVTVKSFDTSLVRKSRTILEAKQEVL

IRESfinder result

IRESfinder score

0.648753455

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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