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mmu_circAtp9b_1329

Basic information of circular RNA
PanCircBase ID:

mmu_circAtp9b_1329

mm39_Chr. Position ID:

chr18|80820012|80952920|-

Gene name:

Atp9b

Transcript:

ENSMUST00000091790.5

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

12

Exon/intron indexes:

15,14,13,12,11,10,9,8,7,6,5,4

Exon/intron offsets:

0,2065,18492,31814,59557,67729,70374,81746,92944,100517,115017,132794

Genomic length:

132908

Exon/intron sizes in nt:

2,49,11,31,43,16,17,77,00,00,00,00,00,00,000

Flanking exon/introns:

chr18:80809147-80820012|chr18:80952920-80956011

Splice length:

1329

Other database IDs:

circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_132317

Human Ortholog IDs:
Mature spliced Sequence:

GTTTTGTATGAACAATTCAAGTTTTTCTTGAATCTCTATTTTCTGGTAGTGTCCTGCTCACAGTTTGTACCAGCATTGAAAATTGGCTACCTCTACACCTACTGGGCTCCTCTGGGATTTGTTTTGGCCGTTACGATTGCACGTGAAGCGATTGATGAATTTCGACGTTTTCAGCGAGACAAGGAAATGAATTCACAGTTATATAGCAAGCTTACAGTAAGAGGTAAAGTTCAAGTTAAGAGCTCAGACATACAAGTTGGAGACCTCATCATAGTGGAAAAGAACCAAAGAATTCCATCAGATATGGTATTTCTTAGAACTTCAGAGAAAGCAGGTTCATGTTTTATCCGCACGGACCAGCTAGATGGTGAGACTGACTGGAAGCTGAAGGTGGCGGTGAGCTGCACACAACGGCTGCCAGCCCTCGGGGACCTTTTTTCTATCAGTGCCTATGTTTATGCTCAGAAGCCACAACTGGATATTCATAGCTTTGAAGGAACATTTACCAGGGAAGACAGTGATCCTCCCATTCATGAGAGTCTCAGCATAGAGAACACTCTGTGGGCAAGCACTATTGTTGCATCAGGTACTGTAATAGGTGTTGTCATTTATACTGGAAAAGAGACTCGAAGTGTAATGAACACATCCAATCCAAAAAATAAGGTTGGTTTATTGGACCTCGAACTCAATCAGCTGACAAAGGCGCTATTTCTGGCTTTAGTTGTTCTTTCTGTTGTTATGGTAACCTTACAGGGATTTGCAGGCCCATGGTACCGTAATCTTTTTCGGTTCCTCCTTCTCTTTTCCTACATCATTCCCATAAGTCTGCGAGTAAACTTGGATATGGGCAAAGCAGCATATGGATGGATGATTATGAAAGATGAGAATATTCCTGGTACAGTTGTTCGGACCAGCACAATACCAGAAGAACTTGGACGCCTGGTGTACTTACTGACAGACAAAACAGGGACGCTCACTCAGAATGAAATGGTATTTAAGCGGCTCCATCTGGGCACTGTCTCCTACGGAACGGACACTATGGACGAAATCCAAAGTCACGTCCTGAATTCCTACCTGCAGGTACACTCACAGCCAAGTGGGCACAACCCAAGCTCTGCTCCACTGAGAAGAAGCCAGTCCTCAACGCCTAAAGTCAAGAAGAGCGTCAGCAGTAGAATCCATGAAGCAGTGAAAGCCATTGCCCTTTGTCATAATGTCACTCCTGTGTATGAGGCTCGGGCTGGCATCACTGGGGAGACTGAGTTTGCTGAAGCAGACCAAGACTTTAGTGATGAGAACCGCACCTACCAGGCCTCCAGCCCCGATGAG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

GCATCACTGGGGAGACTGAG

59.82

TGTGAGCAGGACACTACCAG

59.03

148

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

GCATCACTGGGGAGACTGAG

59.82

CTGTGAGCAGGACACTACCA

59.03

149

PCR template sequence:

TGAGGCTCGGGCTGGCATCACTGGGGAGACTGAGTTTGCTGAAGCAGACCAAGACTTTAGTGATGAGAACCGCACCTACCAGGCCTCCAGCCCCGATGAGGTTTTGTATGAACAATTCAAGTTTTTCTTGAATCTCTATTTTCTGGTAGTGTCCTGCTCACAGTTTGTACCAGCATTGAAAATTGGCTACCTCTACACCT

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

14

Interacting miRNAs (#binding sites)

mmu-miR-9-5p(1), mmu-let-7a-5p(1), mmu-let-7b-5p(1), mmu-miR-135a-5p(1), mmu-miR-101a-3p(1), mmu-let-7c-5p(1), mmu-miR-101b-3p(1), mmu-let-7d-5p(1), mmu-miR-301b-3p(1), mmu-miR-301a-3p(1), mmu-miR-98-5p(1), mmu-miR-1198-5p(1), mmu-miR-452-3p(1), mmu-miR-7a-1-3p(1)

siRNAs for circular RNA silencing
Junction sequence

CAGCCCCGATGAGGTTTTGTATGAAC

siRNA sets

TTCATACAAAACCTCATCG, TCATACAAAACCTCATCGG, GTTCATACAAAACCTCATC

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCATP9B_1329_ORF_1

0.9779

0.13183134

0.999999659

2472

ORF sequence:

ATGAATTCACAGTTATATAGCAAGCTTACAGTAAGAGGTAAAGTTCAAGTTAAGAGCTCAGACATACAAGTTGGAGACCTCATCATAGTGGAAAAGAACCAAAGAATTCCATCAGATATGGTATTTCTTAGAACTTCAGAGAAAGCAGGTTCATGTTTTATCCGCACGGACCAGCTAGATGGTGAGACTGACTGGAAGCTGAAGGTGGCGGTGAGCTGCACACAACGGCTGCCAGCCCTCGGGGACCTTTTTTCTATCAGTGCCTATGTTTATGCTCAGAAGCCACAACTGGATATTCATAGCTTTGAAGGAACATTTACCAGGGAAGACAGTGATCCTCCCATTCATGAGAGTCTCAGCATAGAGAACACTCTGTGGGCAAGCACTATTGTTGCATCAGGTACTGTAATAGGTGTTGTCATTTATACTGGAAAAGAGACTCGAAGTGTAATGAACACATCCAATCCAAAAAATAAGGTTGGTTTATTGGACCTCGAACTCAATCAGCTGACAAAGGCGCTATTTCTGGCTTTAGTTGTTCTTTCTGTTGTTATGGTAACCTTACAGGGATTTGCAGGCCCATGGTACCGTAATCTTTTTCGGTTCCTCCTTCTCTTTTCCTACATCATTCCCATAAGTCTGCGAGTAAACTTGGATATGGGCAAAGCAGCATATGGATGGATGATTATGAAAGATGAGAATATTCCTGGTACAGTTGTTCGGACCAGCACAATACCAGAAGAACTTGGACGCCTGGTGTACTTACTGACAGACAAAACAGGGACGCTCACTCAGAATGAAATGGTATTTAAGCGGCTCCATCTGGGCACTGTCTCCTACGGAACGGACACTATGGACGAAATCCAAAGTCACGTCCTGAATTCCTACCTGCAGGTACACTCACAGCCAAGTGGGCACAACCCAAGCTCTGCTCCACTGAGAAGAAGCCAGTCCTCAACGCCTAAAGTCAAGAAGAGCGTCAGCAGTAGAATCCATGAAGCAGTGAAAGCCATTGCCCTTTGTCATAATGTCACTCCTGTGTATGAGGCTCGGGCTGGCATCACTGGGGAGACTGAGTTTGCTGAAGCAGACCAAGACTTTAGTGATGAGAACCGCACCTACCAGGCCTCCAGCCCCGATGAGGTTTTGTATGAACAATTCAAGTTTTTCTTGAATCTCTATTTTCTGGTAGTGTCCTGCTCACAGTTTGTACCAGCATTGAAAATTGGCTACCTCTACACCTACTGGGCTCCTCTGGGATTTGTTTTGGCCGTTACGATTGCACGTGAAGCGATTGATGAATTTCGACGTTTTCAGCGAGACAAGGAAATGAATTCACAGTTATATAGCAAGCTTACAGTAAGAGGTAAAGTTCAAGTTAAGAGCTCAGACATACAAGTTGGAGACCTCATCATAGTGGAAAAGAACCAAAGAATTCCATCAGATATGGTATTTCTTAGAACTTCAGAGAAAGCAGGTTCATGTTTTATCCGCACGGACCAGCTAGATGGTGAGACTGACTGGAAGCTGAAGGTGGCGGTGAGCTGCACACAACGGCTGCCAGCCCTCGGGGACCTTTTTTCTATCAGTGCCTATGTTTATGCTCAGAAGCCACAACTGGATATTCATAGCTTTGAAGGAACATTTACCAGGGAAGACAGTGATCCTCCCATTCATGAGAGTCTCAGCATAGAGAACACTCTGTGGGCAAGCACTATTGTTGCATCAGGTACTGTAATAGGTGTTGTCATTTATACTGGAAAAGAGACTCGAAGTGTAATGAACACATCCAATCCAAAAAATAAGGTTGGTTTATTGGACCTCGAACTCAATCAGCTGACAAAGGCGCTATTTCTGGCTTTAGTTGTTCTTTCTGTTGTTATGGTAACCTTACAGGGATTTGCAGGCCCATGGTACCGTAATCTTTTTCGGTTCCTCCTTCTCTTTTCCTACATCATTCCCATAAGTCTGCGAGTAAACTTGGATATGGGCAAAGCAGCATATGGATGGATGATTATGAAAGATGAGAATATTCCTGGTACAGTTGTTCGGACCAGCACAATACCAGAAGAACTTGGACGCCTGGTGTACTTACTGACAGACAAAACAGGGACGCTCACTCAGAATGAAATGGTATTTAAGCGGCTCCATCTGGGCACTGTCTCCTACGGAACGGACACTATGGACGAAATCCAAAGTCACGTCCTGAATTCCTACCTGCAGGTACACTCACAGCCAAGTGGGCACAACCCAAGCTCTGCTCCACTGAGAAGAAGCCAGTCCTCAACGCCTAAAGTCAAGAAGAGCGTCAGCAGTAGAATCCATGAAGCAGTGAAAGCCATTGCCCTTTGTCATAATGTCACTCCTGTGTATGAGGCTCGGGCTGGCATCACTGGGGAGACTGAGTTTGCTGAAGCAGACCAAGACTTTAGTGATGAGAACCGCACCTACCAGGCCTCCAGCCCCGATGAG

Peptide sequence:

MNSQLYSKLTVRGKVQVKSSDIQVGDLIIVEKNQRIPSDMVFLRTSEKAGSCFIRTDQLDGETDWKLKVAVSCTQRLPALGDLFSISAYVYAQKPQLDIHSFEGTFTREDSDPPIHESLSIENTLWASTIVASGTVIGVVIYTGKETRSVMNTSNPKNKVGLLDLELNQLTKALFLALVVLSVVMVTLQGFAGPWYRNLFRFLLLFSYIIPISLRVNLDMGKAAYGWMIMKDENIPGTVVRTSTIPEELGRLVYLLTDKTGTLTQNEMVFKRLHLGTVSYGTDTMDEIQSHVLNSYLQVHSQPSGHNPSSAPLRRSQSSTPKVKKSVSSRIHEAVKAIALCHNVTPVYEARAGITGETEFAEADQDFSDENRTYQASSPDEVLYEQFKFFLNLYFLVVSCSQFVPALKIGYLYTYWAPLGFVLAVTIAREAIDEFRRFQRDKEMNSQLYSKLTVRGKVQVKSSDIQVGDLIIVEKNQRIPSDMVFLRTSEKAGSCFIRTDQLDGETDWKLKVAVSCTQRLPALGDLFSISAYVYAQKPQLDIHSFEGTFTREDSDPPIHESLSIENTLWASTIVASGTVIGVVIYTGKETRSVMNTSNPKNKVGLLDLELNQLTKALFLALVVLSVVMVTLQGFAGPWYRNLFRFLLLFSYIIPISLRVNLDMGKAAYGWMIMKDENIPGTVVRTSTIPEELGRLVYLLTDKTGTLTQNEMVFKRLHLGTVSYGTDTMDEIQSHVLNSYLQVHSQPSGHNPSSAPLRRSQSSTPKVKKSVSSRIHEAVKAIALCHNVTPVYEARAGITGETEFAEADQDFSDENRTYQASSPDE

IRESfinder result

IRESfinder score

0.646572287

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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