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mmu_circB3glct_580

Basic information of circular RNA
PanCircBase ID:

mmu_circB3glct_580

mm39_Chr. Position ID:

chr5|149648875|149663066|+

Gene name:

B3glct

Transcript:

ENSMUST00000100404.6

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

6

Exon/intron indexes:

5,6,7,8,9,10

Exon/intron offsets:

0,1343,4513,7262,9959,14121

Genomic length:

14191

Exon/intron sizes in nt:

77,112,137,64,120,70

Flanking exon/introns:

chr5:149632894-149648875|chr5:149663066-149668890

Splice length:

580

Other database IDs:

circAtlas: mmu-B3glct_0010

Human Ortholog IDs:
Mature spliced Sequence:

GATCTCCCTAGAGTCCTCCTTCTCCATCAGTTGGCAAAGCAGGAAGGTGCTTGGACCATCCTTCCCTTGTTACCGCACTTTTCTGTAACATACAGCAAGAATTCAGCGTGGATTTTCTTCTGTGAAGAAGAGACGAGACTACAAATCCCCAGACTCCTAGACACACTCAGACGATATGACCCATCTAAGGAATGGTTTTTAGGAAAAGCACTGTATGACGAAGAGTCGACGATCATCCACCATTACGCCTTCTCTGAGAACCCAACAGTCTTTAAATATCCCGACTTCGCTGCTGGCTGGGCCCTTAGCATCCCGCTGGTGAACAAGCTTGCCAAAAGATTAAAGAGTGAGGCGCTGAAGTCTGACTTCACAATTGATTTAAAGCACGAGATTGCCCTCTACATCTGGGACAAAGGTGGAGGACCGGCCCTCACCCCTGTGCCTGAGTTTTGTACAGAAGATGTGGACCCCCGCTGCGTCACCACGTTCCATTCTTTCCTACCGCTCTGTGGAGTACCGGTAAAGAAGGAGGAGATTTTTGTTGCAGTGAAAACGTGCAAGAAATTTCATGCTGACAGAA

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

TTCCTACCGCTCTGTGGAGT

60.25

GTGCGGTAACAAGGGAAGGA

59.96

163

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

TTCCTACCGCTCTGTGGAGT

60.25

AGTGCGGTAACAAGGGAAGG

59.96

164

PCR template sequence:

ACCACGTTCCATTCTTTCCTACCGCTCTGTGGAGTACCGGTAAAGAAGGAGGAGATTTTTGTTGCAGTGAAAACGTGCAAGAAATTTCATGCTGACAGAAGATCTCCCTAGAGTCCTCCTTCTCCATCAGTTGGCAAAGCAGGAAGGTGCTTGGACCATCCTTCCCTTGTTACCGCACTTTTCTGTAACATACAGCAAGA

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

10

Interacting miRNAs (#binding sites)

mmu-miR-20b-5p(1), mmu-miR-106a-5p(1), mmu-miR-20a-5p(1), mmu-miR-17-5p(1), mmu-miR-106b-5p(1), mmu-miR-24-3p(1), mmu-miR-384-3p(1), mmu-miR-3065-5p(1), mmu-miR-574-5p(1), mmu-miR-376a-3p(1)

siRNAs for circular RNA silencing
Junction sequence

CATGCTGACAGAAGATCTCCCTAGAG

siRNA sets

TCTAGGGAGATCTTCTGTC

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCB3GLCT_580_ORF_1

0.9028

0.18153724

0.804667469

591

ORF sequence:

ATGCTGACAGAAGATCTCCCTAGAGTCCTCCTTCTCCATCAGTTGGCAAAGCAGGAAGGTGCTTGGACCATCCTTCCCTTGTTACCGCACTTTTCTGTAACATACAGCAAGAATTCAGCGTGGATTTTCTTCTGTGAAGAAGAGACGAGACTACAAATCCCCAGACTCCTAGACACACTCAGACGATATGACCCATCTAAGGAATGGTTTTTAGGAAAAGCACTGTATGACGAAGAGTCGACGATCATCCACCATTACGCCTTCTCTGAGAACCCAACAGTCTTTAAATATCCCGACTTCGCTGCTGGCTGGGCCCTTAGCATCCCGCTGGTGAACAAGCTTGCCAAAAGATTAAAGAGTGAGGCGCTGAAGTCTGACTTCACAATTGATTTAAAGCACGAGATTGCCCTCTACATCTGGGACAAAGGTGGAGGACCGGCCCTCACCCCTGTGCCTGAGTTTTGTACAGAAGATGTGGACCCCCGCTGCGTCACCACGTTCCATTCTTTCCTACCGCTCTGTGGAGTACCGGTAAAGAAGGAGGAGATTTTTGTTGCAGTGAAAACGTGCAAGAAATTTCATGCTGACAGA

Peptide sequence:

MLTEDLPRVLLLHQLAKQEGAWTILPLLPHFSVTYSKNSAWIFFCEEETRLQIPRLLDTLRRYDPSKEWFLGKALYDEESTIIHHYAFSENPTVFKYPDFAAGWALSIPLVNKLAKRLKSEALKSDFTIDLKHEIALYIWDKGGGPALTPVPEFCTEDVDPRCVTTFHSFLPLCGVPVKKEEIFVAVKTCKKFHADR

IRESfinder result

IRESfinder score

0.640059421

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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