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mmu_circB3glct_580
Basic information of circular RNA
PanCircBase ID:
mmu_circB3glct_580
mm39_Chr. Position ID:
chr5|149648875|149663066|+
Gene name:
B3glct
Transcript:
ENSMUST00000100404.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
5,6,7,8,9,10
Exon/intron offsets:
0,1343,4513,7262,9959,14121
Genomic length:
14191
Exon/intron sizes in nt:
77,112,137,64,120,70
Flanking exon/introns:
chr5:149632894-149648875|chr5:149663066-149668890
Splice length:
580
Other database IDs:
circAtlas: mmu-B3glct_0010
Human Ortholog IDs:
Mature spliced Sequence:
GATCTCCCTAGAGTCCTCCTTCTCCATCAGTTGGCAAAGCAGGAAGGTGCTTGGACCATCCTTCCCTTGTTACCGCACTTTTCTGTAACATACAGCAAGAATTCAGCGTGGATTTTCTTCTGTGAAGAAGAGACGAGACTACAAATCCCCAGACTCCTAGACACACTCAGACGATATGACCCATCTAAGGAATGGTTTTTAGGAAAAGCACTGTATGACGAAGAGTCGACGATCATCCACCATTACGCCTTCTCTGAGAACCCAACAGTCTTTAAATATCCCGACTTCGCTGCTGGCTGGGCCCTTAGCATCCCGCTGGTGAACAAGCTTGCCAAAAGATTAAAGAGTGAGGCGCTGAAGTCTGACTTCACAATTGATTTAAAGCACGAGATTGCCCTCTACATCTGGGACAAAGGTGGAGGACCGGCCCTCACCCCTGTGCCTGAGTTTTGTACAGAAGATGTGGACCCCCGCTGCGTCACCACGTTCCATTCTTTCCTACCGCTCTGTGGAGTACCGGTAAAGAAGGAGGAGATTTTTGTTGCAGTGAAAACGTGCAAGAAATTTCATGCTGACAGAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTCCTACCGCTCTGTGGAGT
60.25
GTGCGGTAACAAGGGAAGGA
59.96
163
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTCCTACCGCTCTGTGGAGT
60.25
AGTGCGGTAACAAGGGAAGG
59.96
164
PCR template sequence:
ACCACGTTCCATTCTTTCCTACCGCTCTGTGGAGTACCGGTAAAGAAGGAGGAGATTTTTGTTGCAGTGAAAACGTGCAAGAAATTTCATGCTGACAGAAGATCTCCCTAGAGTCCTCCTTCTCCATCAGTTGGCAAAGCAGGAAGGTGCTTGGACCATCCTTCCCTTGTTACCGCACTTTTCTGTAACATACAGCAAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-miR-20b-5p(1), mmu-miR-106a-5p(1), mmu-miR-20a-5p(1), mmu-miR-17-5p(1), mmu-miR-106b-5p(1), mmu-miR-24-3p(1), mmu-miR-384-3p(1), mmu-miR-3065-5p(1), mmu-miR-574-5p(1), mmu-miR-376a-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CATGCTGACAGAAGATCTCCCTAGAG
siRNA sets
TCTAGGGAGATCTTCTGTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCB3GLCT_580_ORF_1
0.9028
0.18153724
0.804667469
591
ORF sequence:
ATGCTGACAGAAGATCTCCCTAGAGTCCTCCTTCTCCATCAGTTGGCAAAGCAGGAAGGTGCTTGGACCATCCTTCCCTTGTTACCGCACTTTTCTGTAACATACAGCAAGAATTCAGCGTGGATTTTCTTCTGTGAAGAAGAGACGAGACTACAAATCCCCAGACTCCTAGACACACTCAGACGATATGACCCATCTAAGGAATGGTTTTTAGGAAAAGCACTGTATGACGAAGAGTCGACGATCATCCACCATTACGCCTTCTCTGAGAACCCAACAGTCTTTAAATATCCCGACTTCGCTGCTGGCTGGGCCCTTAGCATCCCGCTGGTGAACAAGCTTGCCAAAAGATTAAAGAGTGAGGCGCTGAAGTCTGACTTCACAATTGATTTAAAGCACGAGATTGCCCTCTACATCTGGGACAAAGGTGGAGGACCGGCCCTCACCCCTGTGCCTGAGTTTTGTACAGAAGATGTGGACCCCCGCTGCGTCACCACGTTCCATTCTTTCCTACCGCTCTGTGGAGTACCGGTAAAGAAGGAGGAGATTTTTGTTGCAGTGAAAACGTGCAAGAAATTTCATGCTGACAGA
Peptide sequence:
MLTEDLPRVLLLHQLAKQEGAWTILPLLPHFSVTYSKNSAWIFFCEEETRLQIPRLLDTLRRYDPSKEWFLGKALYDEESTIIHHYAFSENPTVFKYPDFAAGWALSIPLVNKLAKRLKSEALKSDFTIDLKHEIALYIWDKGGGPALTPVPEFCTEDVDPRCVTTFHSFLPLCGVPVKKEEIFVAVKTCKKFHADR
IRESfinder result
IRESfinder score
0.640059421
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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