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mmu_circBach2_1590

Basic information of circular RNA
PanCircBase ID:

mmu_circBach2_1590

mm39_Chr. Position ID:

chr4|32561777|32563367|+

Gene name:

Bach2

Transcript:

ENSMUST00000108180.9

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

1

Exon/intron indexes:

5

Exon/intron offsets:

0

Genomic length:

1590

Exon/intron sizes in nt:

1590

Flanking exon/introns:

chr4:32501867-32561777|chr4:32563367-32575239

Splice length:

1590

Other database IDs:

circBase: mmu_circ_0011556, CIRCpedia: MMU_CIRCpedia_37371, circAtlas: mmu-Bach2_0003

circBase: hsa_circ_0132668, circAtlas: hsa-BACH2_0003, CIRCpedia: HSA_CIRCpedia_356423

Human Ortholog IDs:
Mature spliced Sequence:

GTCACGGCCAGGGGCTTTGGGCCACTGCTACAGTTTGCCTACACTGCCAAGCTGTTACTCAGCAGAGAGAACATCCGGGAGGTCATCCGCTGTGCTGAGTTCCTGCGCATGCACAATCTGGAGGACTCCTGCTTCAGCTTCCTGCAGACCCAGCTCCTGAACAGGGAGGATGGCCTGTTTGTTTGCAGAAAGGACAGTGCGTGCCAGCGCCCCCAAGAGGACCATGGGAATTCTGCAGGAGAAGAGGAGGAAGAAGAGGAAACAATGGACTCAGAGACAGCTAGGATGGCTTGCGCCACAGACCAGATGCTCCCAGACCCCATCAGCTTTGAGGCCACTGCCATCCCAGTAGCAGAGAAAGAAGAAGCCCTGCTCCCTGAGTCTGAGGTACCCACAGACACCAAGGAAAACTCAGAAAAGGGTGCATTGACCCAGTACCCTAGATATAAGAAGTACCAGCTCGCGTGTACCAAGAATGTCTACAGTGCACCCTCACACGGTACCTCAGGGTTTGCGAGCACATTCAGTGAAGACAGTCCTGGCAATAGCCTCAAGCCGGGGCTCCCCATGGGACAGATTAAAAGTGAGCCACCCAGTGAGGAGACCGAGGAGGAGAGCATCACCCTCTGCCTGTCCGGAGATGAGACCGACATCAAAGACAGACCAGGAGATGTCGAGATGGACCGAAAACAACCCAGCCCTGCCCCTACCCCCAGTACCCCTACTGGAGCCGCCTGCCTGGACAGGTCCAGGAGTGTGTCCTCGCCTTCCTGCCTGCGGTCTCTGTTCGGTATAACGAAAGGTGTGGAGTCCACTGGCCTGCCCAGTACATCTCAGCAACCCTTAGTCAGGAGCTCAGCGTGCCCTTTCAACAAGGGGATCTCTCAGGGTGACCTTAAAACTGATTACACCCCTTTGGCAGGGAATTACGGACAGCCCCACGTGGGCCAGAAGGATGTGTCCAACTTCGCAATGGGGTCGCCACTCAGGGGGCCCGGGCCGGAGACTCTCTGTAAGCAGGAGGGAGAGCTGGACCGCCGGAGCGTCATCTTCTCCGCAAGCGCGTGTGACCAACCGAACACTCCAGTGCATTCATACTCTGCGGTGAGCAATTTGGACAAAGACCTTTCGGAGCCGGTGCCAAAAAGTCTATGGGTGGGAGCTGGCCAGTCCCTCCCCAGCTCCCAGGCTTACTCCCACAGCGGACTGATGGCTGACCACTTGCCAGGAAGGATACGGCCCAACACCAGCTGCCCGGTGCCAATCAAAGTCTGTCCTCGCTCACCTCCGCTCGAGACCAGGACTAGGACCTCCAGCTCCTGCTCCTCGTATTCCTACGCAGAGGACGGGAGTGGGGGCTCCCCCTGCAGCCTCCCTCTCTGTGAGTTCTCCTCTTCGCCCTGTTCCCAGGGAGCCAGATTCCTTGCCACGGAACATCAGGAACCAGGCCTGATGGGAGATGGAATGTACAACCAAGTCCGACCCCAGATTAAATGTGAGCAGTCTTACGGAACCAATTCCAGTGACGAGTCTGGATCATTCTCGGAAGCAGACAGTGAGTCGTGTCCTGTGCAGGACAGGGGCCAGGAG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

GTGAGCAGTCTTACGGAACCA

60

TGACCTCCCGGATGTTCTCT

59.96

180

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

GTGAGCAGTCTTACGGAACCA

60

CTCCCGGATGTTCTCTCTGC

59.9

176

PCR template sequence:

TAAATGTGAGCAGTCTTACGGAACCAATTCCAGTGACGAGTCTGGATCATTCTCGGAAGCAGACAGTGAGTCGTGTCCTGTGCAGGACAGGGGCCAGGAGGTCACGGCCAGGGGCTTTGGGCCACTGCTACAGTTTGCCTACACTGCCAAGCTGTTACTCAGCAGAGAGAACATCCGGGAGGTCATCCGCTGTGCTGAGT

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

17

Interacting miRNAs (#binding sites)

mmu-miR-210-3p(1), mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-298-5p(1), mmu-miR-125b-5p(1), mmu-miR-138-5p(1), mmu-miR-339-5p(1), mmu-miR-495-3p(1), mmu-miR-149-5p(1), mmu-miR-351-5p(1), mmu-miR-125a-5p(1), mmu-miR-96-5p(1), mmu-miR-34b-5p(1), mmu-miR-705(1), mmu-miR-207(1), mmu-miR-697(1), mmu-miR-574-5p(1)

siRNAs for circular RNA silencing
Junction sequence

CAGGGGCCAGGAGGTCACGGCCAGGG

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCBACH2_1590_ORF_1

0.8234

0.276939086

0.999999997

3072

ORF sequence:

ATGCACAATCTGGAGGACTCCTGCTTCAGCTTCCTGCAGACCCAGCTCCTGAACAGGGAGGATGGCCTGTTTGTTTGCAGAAAGGACAGTGCGTGCCAGCGCCCCCAAGAGGACCATGGGAATTCTGCAGGAGAAGAGGAGGAAGAAGAGGAAACAATGGACTCAGAGACAGCTAGGATGGCTTGCGCCACAGACCAGATGCTCCCAGACCCCATCAGCTTTGAGGCCACTGCCATCCCAGTAGCAGAGAAAGAAGAAGCCCTGCTCCCTGAGTCTGAGGTACCCACAGACACCAAGGAAAACTCAGAAAAGGGTGCATTGACCCAGTACCCTAGATATAAGAAGTACCAGCTCGCGTGTACCAAGAATGTCTACAGTGCACCCTCACACGGTACCTCAGGGTTTGCGAGCACATTCAGTGAAGACAGTCCTGGCAATAGCCTCAAGCCGGGGCTCCCCATGGGACAGATTAAAAGTGAGCCACCCAGTGAGGAGACCGAGGAGGAGAGCATCACCCTCTGCCTGTCCGGAGATGAGACCGACATCAAAGACAGACCAGGAGATGTCGAGATGGACCGAAAACAACCCAGCCCTGCCCCTACCCCCAGTACCCCTACTGGAGCCGCCTGCCTGGACAGGTCCAGGAGTGTGTCCTCGCCTTCCTGCCTGCGGTCTCTGTTCGGTATAACGAAAGGTGTGGAGTCCACTGGCCTGCCCAGTACATCTCAGCAACCCTTAGTCAGGAGCTCAGCGTGCCCTTTCAACAAGGGGATCTCTCAGGGTGACCTTAAAACTGATTACACCCCTTTGGCAGGGAATTACGGACAGCCCCACGTGGGCCAGAAGGATGTGTCCAACTTCGCAATGGGGTCGCCACTCAGGGGGCCCGGGCCGGAGACTCTCTGTAAGCAGGAGGGAGAGCTGGACCGCCGGAGCGTCATCTTCTCCGCAAGCGCGTGTGACCAACCGAACACTCCAGTGCATTCATACTCTGCGGTGAGCAATTTGGACAAAGACCTTTCGGAGCCGGTGCCAAAAAGTCTATGGGTGGGAGCTGGCCAGTCCCTCCCCAGCTCCCAGGCTTACTCCCACAGCGGACTGATGGCTGACCACTTGCCAGGAAGGATACGGCCCAACACCAGCTGCCCGGTGCCAATCAAAGTCTGTCCTCGCTCACCTCCGCTCGAGACCAGGACTAGGACCTCCAGCTCCTGCTCCTCGTATTCCTACGCAGAGGACGGGAGTGGGGGCTCCCCCTGCAGCCTCCCTCTCTGTGAGTTCTCCTCTTCGCCCTGTTCCCAGGGAGCCAGATTCCTTGCCACGGAACATCAGGAACCAGGCCTGATGGGAGATGGAATGTACAACCAAGTCCGACCCCAGATTAAATGTGAGCAGTCTTACGGAACCAATTCCAGTGACGAGTCTGGATCATTCTCGGAAGCAGACAGTGAGTCGTGTCCTGTGCAGGACAGGGGCCAGGAGGTCACGGCCAGGGGCTTTGGGCCACTGCTACAGTTTGCCTACACTGCCAAGCTGTTACTCAGCAGAGAGAACATCCGGGAGGTCATCCGCTGTGCTGAGTTCCTGCGCATGCACAATCTGGAGGACTCCTGCTTCAGCTTCCTGCAGACCCAGCTCCTGAACAGGGAGGATGGCCTGTTTGTTTGCAGAAAGGACAGTGCGTGCCAGCGCCCCCAAGAGGACCATGGGAATTCTGCAGGAGAAGAGGAGGAAGAAGAGGAAACAATGGACTCAGAGACAGCTAGGATGGCTTGCGCCACAGACCAGATGCTCCCAGACCCCATCAGCTTTGAGGCCACTGCCATCCCAGTAGCAGAGAAAGAAGAAGCCCTGCTCCCTGAGTCTGAGGTACCCACAGACACCAAGGAAAACTCAGAAAAGGGTGCATTGACCCAGTACCCTAGATATAAGAAGTACCAGCTCGCGTGTACCAAGAATGTCTACAGTGCACCCTCACACGGTACCTCAGGGTTTGCGAGCACATTCAGTGAAGACAGTCCTGGCAATAGCCTCAAGCCGGGGCTCCCCATGGGACAGATTAAAAGTGAGCCACCCAGTGAGGAGACCGAGGAGGAGAGCATCACCCTCTGCCTGTCCGGAGATGAGACCGACATCAAAGACAGACCAGGAGATGTCGAGATGGACCGAAAACAACCCAGCCCTGCCCCTACCCCCAGTACCCCTACTGGAGCCGCCTGCCTGGACAGGTCCAGGAGTGTGTCCTCGCCTTCCTGCCTGCGGTCTCTGTTCGGTATAACGAAAGGTGTGGAGTCCACTGGCCTGCCCAGTACATCTCAGCAACCCTTAGTCAGGAGCTCAGCGTGCCCTTTCAACAAGGGGATCTCTCAGGGTGACCTTAAAACTGATTACACCCCTTTGGCAGGGAATTACGGACAGCCCCACGTGGGCCAGAAGGATGTGTCCAACTTCGCAATGGGGTCGCCACTCAGGGGGCCCGGGCCGGAGACTCTCTGTAAGCAGGAGGGAGAGCTGGACCGCCGGAGCGTCATCTTCTCCGCAAGCGCGTGTGACCAACCGAACACTCCAGTGCATTCATACTCTGCGGTGAGCAATTTGGACAAAGACCTTTCGGAGCCGGTGCCAAAAAGTCTATGGGTGGGAGCTGGCCAGTCCCTCCCCAGCTCCCAGGCTTACTCCCACAGCGGACTGATGGCTGACCACTTGCCAGGAAGGATACGGCCCAACACCAGCTGCCCGGTGCCAATCAAAGTCTGTCCTCGCTCACCTCCGCTCGAGACCAGGACTAGGACCTCCAGCTCCTGCTCCTCGTATTCCTACGCAGAGGACGGGAGTGGGGGCTCCCCCTGCAGCCTCCCTCTCTGTGAGTTCTCCTCTTCGCCCTGTTCCCAGGGAGCCAGATTCCTTGCCACGGAACATCAGGAACCAGGCCTGATGGGAGATGGAATGTACAACCAAGTCCGACCCCAGATTAAATGTGAGCAGTCTTACGGAACCAATTCCAGTGACGAGTCTGGATCATTCTCGGAAGCAGACAGTGAGTCGTGTCCTGTGCAGGACAGGGGCCAGGAG

Peptide sequence:

MHNLEDSCFSFLQTQLLNREDGLFVCRKDSACQRPQEDHGNSAGEEEEEEETMDSETARMACATDQMLPDPISFEATAIPVAEKEEALLPESEVPTDTKENSEKGALTQYPRYKKYQLACTKNVYSAPSHGTSGFASTFSEDSPGNSLKPGLPMGQIKSEPPSEETEEESITLCLSGDETDIKDRPGDVEMDRKQPSPAPTPSTPTGAACLDRSRSVSSPSCLRSLFGITKGVESTGLPSTSQQPLVRSSACPFNKGISQGDLKTDYTPLAGNYGQPHVGQKDVSNFAMGSPLRGPGPETLCKQEGELDRRSVIFSASACDQPNTPVHSYSAVSNLDKDLSEPVPKSLWVGAGQSLPSSQAYSHSGLMADHLPGRIRPNTSCPVPIKVCPRSPPLETRTRTSSSCSSYSYAEDGSGGSPCSLPLCEFSSSPCSQGARFLATEHQEPGLMGDGMYNQVRPQIKCEQSYGTNSSDESGSFSEADSESCPVQDRGQEVTARGFGPLLQFAYTAKLLLSRENIREVIRCAEFLRMHNLEDSCFSFLQTQLLNREDGLFVCRKDSACQRPQEDHGNSAGEEEEEEETMDSETARMACATDQMLPDPISFEATAIPVAEKEEALLPESEVPTDTKENSEKGALTQYPRYKKYQLACTKNVYSAPSHGTSGFASTFSEDSPGNSLKPGLPMGQIKSEPPSEETEEESITLCLSGDETDIKDRPGDVEMDRKQPSPAPTPSTPTGAACLDRSRSVSSPSCLRSLFGITKGVESTGLPSTSQQPLVRSSACPFNKGISQGDLKTDYTPLAGNYGQPHVGQKDVSNFAMGSPLRGPGPETLCKQEGELDRRSVIFSASACDQPNTPVHSYSAVSNLDKDLSEPVPKSLWVGAGQSLPSSQAYSHSGLMADHLPGRIRPNTSCPVPIKVCPRSPPLETRTRTSSSCSSYSYAEDGSGGSPCSLPLCEFSSSPCSQGARFLATEHQEPGLMGDGMYNQVRPQIKCEQSYGTNSSDESGSFSEADSESCPVQDRGQE

IRESfinder result

IRESfinder score

0.426220905

IRES/non IRES

non-IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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