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mmu_circBbs2_1714
Basic information of circular RNA
PanCircBase ID:
mmu_circBbs2_1714
mm39_Chr. Position ID:
chr8|94796589|94816515|-
Gene name:
Bbs2
Transcript:
ENSMUST00000034206.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
14
Exon/intron indexes:
16,15,14,13,12,11,10,9,8,7,6,5,4,3
Exon/intron offsets:
0,4330,5161,6990,9521,10806,11063,12036,12394,16694,16869,17415,19141,19800
Genomic length:
19926
Exon/intron sizes in nt:
14,91,13,13,81,32,13,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr8:94795010-94796589|chr8:94816515-94819022
Splice length:
1714
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTCTCAGATGGGGCAAATGCCATTGTGCTGGGGACCCTGGGAGACATTGCCCCACCTCTTGCAATCATCGGTGGAAACTGTGCTCTGCAGGGTTTCGATCACGAAGGAAATGATCTCTTTTGGACGGTTACTGGAGACAATGTGCATTCCTTGGCTCTGTGTGACTTTGACGGTGATGGGAAGACAGAGCTTCTTGTTGGATCTGAAGATTTTGACATTCGAGTTTTTAAAGAAGATGAGATTGTGGCAGAAATGACAGAGACAGAGATAGTCACCTCTCTGTGTCCTATGTATGGCAGTCGGTTTGGTTACGCCCTTTCTAATGGCACAGTTGGAGTTTATGACAAAACAGCCCGGTACTGGAGAATTAAATCCAAAAACCATGCCATGAGCATCCATGCTTTTGACATTAATTCTGATGGAGTGTGTGAACTGATAACCGGGTGGTCTAATGGGAAGGTTGATGCTCGCAGTGACCGGACTGGAGAGGTCATATTTAAAGACAACTTCTCTTCTGCTGTTGCTGGTGTGGTAGAGGGAGATTACCGGATGGATGGCCACGTACAGTTAATCTGCTGCTCAGTGGATGGGGAAATCCGAGGCTACCTGCCAGGCACAGCTGAGATGAAGGGGAACCTCCTGGACACAAGTGTGGAGCAGGACCTGATCCGAGAGCTGAGTCAGAAAAAGCAGAACCTGTTGCTAGAACTTCGCAACTATGAGGAGAGCACCAAGGCAGAACTGAGCAGTCCCCTGAACGAGGCCGATGGGCAGAAAGGCATAATCCCAGCCAATACCAGGCTCCACACAGCGCTCTCAGTCAATATGGGGAATGACTTACAAGATGCGCATGCGGAATTAGGCATTTCCACTTCCAATGATACTATCATCCGAGCAGTACTAATTTTTGCAGAGGGAATTTTTGTGGGTGAAAGCCATGTGGTCCACCCCAGCATTCACAACCTTTCCAGCTCCCTCCGGGTACCAATTACACCTCCCAAAGATGTCCCTGTGGATCTGCACCTGAAAACCTTCGTGGGTTACAGAAGCAGCACCCAGTTCCATGTGTTTGAACTGACAAGACAGCTGCCCCGATTCACCATGTATGCATTGACAAGCCCAGATGCAGCTAGCGAGCCTGTCAGCTACGTGAACTTCAGTGTTGCCGAGCGCACACAGAGGATGGTCACGTGGCTCAACCAGAACTTTCTGTTGCCAGAAGACAGTAACGTTCAGAATTCCCCGTTTCACGTCTGTTTCACATCGTTACGCAATGGTGGCCAGCTCTACATAAAAATGAAACAAAGTGGTGAGATCACTGTGAATACTGACGATATTGATCTAGCTGGTGACATCATCCAGTCGATCGCGTCCTTTTTTGCTATTGAAGACCTTCAGGTAGAAGCAGATTTCCCTGTGTACTTCGAGGAACTACGGAAAGTGCTGGTGAAGGTGGATGAGTATCATTCAGTGCATCAAAAGCTCAGCGCCGACATGGCTGATAATTCTAACCTCATCCGAAGTTTGCTGGTCAGAGCGGAGGATGCTCGGCTGATGAGGGACATGAAAACAATGAAGAGTCGTTACATGGAGCTCTATGACCTTAATAAAGATTTGCTGAATGGATACAAGATTCGCTGTAACAACCACACAGAACTGCTAGGAAACCTGAAGGCGGTGAACCAAGCAATCCAAAGAGCAGGCCGTCTGCGCG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGCTAGGAAACCTGAAGGCG
60.04
AACCCTGCAGAGCACAGTTT
60.11
151
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGCTAGGAAACCTGAAGGCG
60.04
AAACCCTGCAGAGCACAGTT
60.11
152
PCR template sequence:
TTGCTGAATGGATACAAGATTCGCTGTAACAACCACACAGAACTGCTAGGAAACCTGAAGGCGGTGAACCAAGCAATCCAAAGAGCAGGCCGTCTGCGCGGTCTCAGATGGGGCAAATGCCATTGTGCTGGGGACCCTGGGAGACATTGCCCCACCTCTTGCAATCATCGGTGGAAACTGTGCTCTGCAGGGTTTCGATC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
18
Interacting miRNAs (#binding sites)
mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-27b-3p(1), mmu-miR-135a-5p(1), mmu-miR-223-3p(1), mmu-miR-205-5p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-298-5p(1), mmu-miR-10b-5p(1), mmu-miR-10a-5p(1), mmu-miR-128-3p(1), mmu-miR-503-5p(1), mmu-miR-466d-3p(1), mmu-miR-384-3p(1), mmu-miR-377-3p(1), mmu-miR-335-3p(1), mmu-miR-452-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GGCCGTCTGCGCGGTCTCAGATGGGG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBBS2_1714_ORF_1
1.0341
0.26955083
0.999884674
1575
ORF sequence:
ATGACAGAGACAGAGATAGTCACCTCTCTGTGTCCTATGTATGGCAGTCGGTTTGGTTACGCCCTTTCTAATGGCACAGTTGGAGTTTATGACAAAACAGCCCGGTACTGGAGAATTAAATCCAAAAACCATGCCATGAGCATCCATGCTTTTGACATTAATTCTGATGGAGTGTGTGAACTGATAACCGGGTGGTCTAATGGGAAGGTTGATGCTCGCAGTGACCGGACTGGAGAGGTCATATTTAAAGACAACTTCTCTTCTGCTGTTGCTGGTGTGGTAGAGGGAGATTACCGGATGGATGGCCACGTACAGTTAATCTGCTGCTCAGTGGATGGGGAAATCCGAGGCTACCTGCCAGGCACAGCTGAGATGAAGGGGAACCTCCTGGACACAAGTGTGGAGCAGGACCTGATCCGAGAGCTGAGTCAGAAAAAGCAGAACCTGTTGCTAGAACTTCGCAACTATGAGGAGAGCACCAAGGCAGAACTGAGCAGTCCCCTGAACGAGGCCGATGGGCAGAAAGGCATAATCCCAGCCAATACCAGGCTCCACACAGCGCTCTCAGTCAATATGGGGAATGACTTACAAGATGCGCATGCGGAATTAGGCATTTCCACTTCCAATGATACTATCATCCGAGCAGTACTAATTTTTGCAGAGGGAATTTTTGTGGGTGAAAGCCATGTGGTCCACCCCAGCATTCACAACCTTTCCAGCTCCCTCCGGGTACCAATTACACCTCCCAAAGATGTCCCTGTGGATCTGCACCTGAAAACCTTCGTGGGTTACAGAAGCAGCACCCAGTTCCATGTGTTTGAACTGACAAGACAGCTGCCCCGATTCACCATGTATGCATTGACAAGCCCAGATGCAGCTAGCGAGCCTGTCAGCTACGTGAACTTCAGTGTTGCCGAGCGCACACAGAGGATGGTCACGTGGCTCAACCAGAACTTTCTGTTGCCAGAAGACAGTAACGTTCAGAATTCCCCGTTTCACGTCTGTTTCACATCGTTACGCAATGGTGGCCAGCTCTACATAAAAATGAAACAAAGTGGTGAGATCACTGTGAATACTGACGATATTGATCTAGCTGGTGACATCATCCAGTCGATCGCGTCCTTTTTTGCTATTGAAGACCTTCAGGTAGAAGCAGATTTCCCTGTGTACTTCGAGGAACTACGGAAAGTGCTGGTGAAGGTGGATGAGTATCATTCAGTGCATCAAAAGCTCAGCGCCGACATGGCTGATAATTCTAACCTCATCCGAAGTTTGCTGGTCAGAGCGGAGGATGCTCGGCTGATGAGGGACATGAAAACAATGAAGAGTCGTTACATGGAGCTCTATGACCTTAATAAAGATTTGCTGAATGGATACAAGATTCGCTGTAACAACCACACAGAACTGCTAGGAAACCTGAAGGCGGTGAACCAAGCAATCCAAAGAGCAGGCCGTCTGCGCGGTCTCAGATGGGGCAAATGCCATTGTGCTGGGGACCCTGGGAGACATTGCCCCACCTCTTGCAATCATCGGTGGAAACTGTGCTCTGCAGGGTTTCGATCACGAAGGAAATGA
Peptide sequence:
MTETEIVTSLCPMYGSRFGYALSNGTVGVYDKTARYWRIKSKNHAMSIHAFDINSDGVCELITGWSNGKVDARSDRTGEVIFKDNFSSAVAGVVEGDYRMDGHVQLICCSVDGEIRGYLPGTAEMKGNLLDTSVEQDLIRELSQKKQNLLLELRNYEESTKAELSSPLNEADGQKGIIPANTRLHTALSVNMGNDLQDAHAELGISTSNDTIIRAVLIFAEGIFVGESHVVHPSIHNLSSSLRVPITPPKDVPVDLHLKTFVGYRSSTQFHVFELTRQLPRFTMYALTSPDAASEPVSYVNFSVAERTQRMVTWLNQNFLLPEDSNVQNSPFHVCFTSLRNGGQLYIKMKQSGEITVNTDDIDLAGDIIQSIASFFAIEDLQVEADFPVYFEELRKVLVKVDEYHSVHQKLSADMADNSNLIRSLLVRAEDARLMRDMKTMKSRYMELYDLNKDLLNGYKIRCNNHTELLGNLKAVNQAIQRAGRLRGLRWGKCHCAGDPGRHCPTSCNHRWKLCSAGFRSRRK
IRESfinder result
IRESfinder score
0.453492372
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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