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mmu_circBbs2_1714

Basic information of circular RNA
PanCircBase ID:

mmu_circBbs2_1714

mm39_Chr. Position ID:

chr8|94796589|94816515|-

Gene name:

Bbs2

Transcript:

ENSMUST00000034206.6

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

14

Exon/intron indexes:

16,15,14,13,12,11,10,9,8,7,6,5,4,3

Exon/intron offsets:

0,4330,5161,6990,9521,10806,11063,12036,12394,16694,16869,17415,19141,19800

Genomic length:

19926

Exon/intron sizes in nt:

14,91,13,13,81,32,13,00,00,00,00,00,00,00,00,00,00,00,000

Flanking exon/introns:

chr8:94795010-94796589|chr8:94816515-94819022

Splice length:

1714

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GTCTCAGATGGGGCAAATGCCATTGTGCTGGGGACCCTGGGAGACATTGCCCCACCTCTTGCAATCATCGGTGGAAACTGTGCTCTGCAGGGTTTCGATCACGAAGGAAATGATCTCTTTTGGACGGTTACTGGAGACAATGTGCATTCCTTGGCTCTGTGTGACTTTGACGGTGATGGGAAGACAGAGCTTCTTGTTGGATCTGAAGATTTTGACATTCGAGTTTTTAAAGAAGATGAGATTGTGGCAGAAATGACAGAGACAGAGATAGTCACCTCTCTGTGTCCTATGTATGGCAGTCGGTTTGGTTACGCCCTTTCTAATGGCACAGTTGGAGTTTATGACAAAACAGCCCGGTACTGGAGAATTAAATCCAAAAACCATGCCATGAGCATCCATGCTTTTGACATTAATTCTGATGGAGTGTGTGAACTGATAACCGGGTGGTCTAATGGGAAGGTTGATGCTCGCAGTGACCGGACTGGAGAGGTCATATTTAAAGACAACTTCTCTTCTGCTGTTGCTGGTGTGGTAGAGGGAGATTACCGGATGGATGGCCACGTACAGTTAATCTGCTGCTCAGTGGATGGGGAAATCCGAGGCTACCTGCCAGGCACAGCTGAGATGAAGGGGAACCTCCTGGACACAAGTGTGGAGCAGGACCTGATCCGAGAGCTGAGTCAGAAAAAGCAGAACCTGTTGCTAGAACTTCGCAACTATGAGGAGAGCACCAAGGCAGAACTGAGCAGTCCCCTGAACGAGGCCGATGGGCAGAAAGGCATAATCCCAGCCAATACCAGGCTCCACACAGCGCTCTCAGTCAATATGGGGAATGACTTACAAGATGCGCATGCGGAATTAGGCATTTCCACTTCCAATGATACTATCATCCGAGCAGTACTAATTTTTGCAGAGGGAATTTTTGTGGGTGAAAGCCATGTGGTCCACCCCAGCATTCACAACCTTTCCAGCTCCCTCCGGGTACCAATTACACCTCCCAAAGATGTCCCTGTGGATCTGCACCTGAAAACCTTCGTGGGTTACAGAAGCAGCACCCAGTTCCATGTGTTTGAACTGACAAGACAGCTGCCCCGATTCACCATGTATGCATTGACAAGCCCAGATGCAGCTAGCGAGCCTGTCAGCTACGTGAACTTCAGTGTTGCCGAGCGCACACAGAGGATGGTCACGTGGCTCAACCAGAACTTTCTGTTGCCAGAAGACAGTAACGTTCAGAATTCCCCGTTTCACGTCTGTTTCACATCGTTACGCAATGGTGGCCAGCTCTACATAAAAATGAAACAAAGTGGTGAGATCACTGTGAATACTGACGATATTGATCTAGCTGGTGACATCATCCAGTCGATCGCGTCCTTTTTTGCTATTGAAGACCTTCAGGTAGAAGCAGATTTCCCTGTGTACTTCGAGGAACTACGGAAAGTGCTGGTGAAGGTGGATGAGTATCATTCAGTGCATCAAAAGCTCAGCGCCGACATGGCTGATAATTCTAACCTCATCCGAAGTTTGCTGGTCAGAGCGGAGGATGCTCGGCTGATGAGGGACATGAAAACAATGAAGAGTCGTTACATGGAGCTCTATGACCTTAATAAAGATTTGCTGAATGGATACAAGATTCGCTGTAACAACCACACAGAACTGCTAGGAAACCTGAAGGCGGTGAACCAAGCAATCCAAAGAGCAGGCCGTCTGCGCG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

TGCTAGGAAACCTGAAGGCG

60.04

AACCCTGCAGAGCACAGTTT

60.11

151

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

TGCTAGGAAACCTGAAGGCG

60.04

AAACCCTGCAGAGCACAGTT

60.11

152

PCR template sequence:

TTGCTGAATGGATACAAGATTCGCTGTAACAACCACACAGAACTGCTAGGAAACCTGAAGGCGGTGAACCAAGCAATCCAAAGAGCAGGCCGTCTGCGCGGTCTCAGATGGGGCAAATGCCATTGTGCTGGGGACCCTGGGAGACATTGCCCCACCTCTTGCAATCATCGGTGGAAACTGTGCTCTGCAGGGTTTCGATC

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

18

Interacting miRNAs (#binding sites)

mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-27b-3p(1), mmu-miR-135a-5p(1), mmu-miR-223-3p(1), mmu-miR-205-5p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-298-5p(1), mmu-miR-10b-5p(1), mmu-miR-10a-5p(1), mmu-miR-128-3p(1), mmu-miR-503-5p(1), mmu-miR-466d-3p(1), mmu-miR-384-3p(1), mmu-miR-377-3p(1), mmu-miR-335-3p(1), mmu-miR-452-3p(1)

siRNAs for circular RNA silencing
Junction sequence

GGCCGTCTGCGCGGTCTCAGATGGGG

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCBBS2_1714_ORF_1

1.0341

0.26955083

0.999884674

1575

ORF sequence:

ATGACAGAGACAGAGATAGTCACCTCTCTGTGTCCTATGTATGGCAGTCGGTTTGGTTACGCCCTTTCTAATGGCACAGTTGGAGTTTATGACAAAACAGCCCGGTACTGGAGAATTAAATCCAAAAACCATGCCATGAGCATCCATGCTTTTGACATTAATTCTGATGGAGTGTGTGAACTGATAACCGGGTGGTCTAATGGGAAGGTTGATGCTCGCAGTGACCGGACTGGAGAGGTCATATTTAAAGACAACTTCTCTTCTGCTGTTGCTGGTGTGGTAGAGGGAGATTACCGGATGGATGGCCACGTACAGTTAATCTGCTGCTCAGTGGATGGGGAAATCCGAGGCTACCTGCCAGGCACAGCTGAGATGAAGGGGAACCTCCTGGACACAAGTGTGGAGCAGGACCTGATCCGAGAGCTGAGTCAGAAAAAGCAGAACCTGTTGCTAGAACTTCGCAACTATGAGGAGAGCACCAAGGCAGAACTGAGCAGTCCCCTGAACGAGGCCGATGGGCAGAAAGGCATAATCCCAGCCAATACCAGGCTCCACACAGCGCTCTCAGTCAATATGGGGAATGACTTACAAGATGCGCATGCGGAATTAGGCATTTCCACTTCCAATGATACTATCATCCGAGCAGTACTAATTTTTGCAGAGGGAATTTTTGTGGGTGAAAGCCATGTGGTCCACCCCAGCATTCACAACCTTTCCAGCTCCCTCCGGGTACCAATTACACCTCCCAAAGATGTCCCTGTGGATCTGCACCTGAAAACCTTCGTGGGTTACAGAAGCAGCACCCAGTTCCATGTGTTTGAACTGACAAGACAGCTGCCCCGATTCACCATGTATGCATTGACAAGCCCAGATGCAGCTAGCGAGCCTGTCAGCTACGTGAACTTCAGTGTTGCCGAGCGCACACAGAGGATGGTCACGTGGCTCAACCAGAACTTTCTGTTGCCAGAAGACAGTAACGTTCAGAATTCCCCGTTTCACGTCTGTTTCACATCGTTACGCAATGGTGGCCAGCTCTACATAAAAATGAAACAAAGTGGTGAGATCACTGTGAATACTGACGATATTGATCTAGCTGGTGACATCATCCAGTCGATCGCGTCCTTTTTTGCTATTGAAGACCTTCAGGTAGAAGCAGATTTCCCTGTGTACTTCGAGGAACTACGGAAAGTGCTGGTGAAGGTGGATGAGTATCATTCAGTGCATCAAAAGCTCAGCGCCGACATGGCTGATAATTCTAACCTCATCCGAAGTTTGCTGGTCAGAGCGGAGGATGCTCGGCTGATGAGGGACATGAAAACAATGAAGAGTCGTTACATGGAGCTCTATGACCTTAATAAAGATTTGCTGAATGGATACAAGATTCGCTGTAACAACCACACAGAACTGCTAGGAAACCTGAAGGCGGTGAACCAAGCAATCCAAAGAGCAGGCCGTCTGCGCGGTCTCAGATGGGGCAAATGCCATTGTGCTGGGGACCCTGGGAGACATTGCCCCACCTCTTGCAATCATCGGTGGAAACTGTGCTCTGCAGGGTTTCGATCACGAAGGAAATGA

Peptide sequence:

MTETEIVTSLCPMYGSRFGYALSNGTVGVYDKTARYWRIKSKNHAMSIHAFDINSDGVCELITGWSNGKVDARSDRTGEVIFKDNFSSAVAGVVEGDYRMDGHVQLICCSVDGEIRGYLPGTAEMKGNLLDTSVEQDLIRELSQKKQNLLLELRNYEESTKAELSSPLNEADGQKGIIPANTRLHTALSVNMGNDLQDAHAELGISTSNDTIIRAVLIFAEGIFVGESHVVHPSIHNLSSSLRVPITPPKDVPVDLHLKTFVGYRSSTQFHVFELTRQLPRFTMYALTSPDAASEPVSYVNFSVAERTQRMVTWLNQNFLLPEDSNVQNSPFHVCFTSLRNGGQLYIKMKQSGEITVNTDDIDLAGDIIQSIASFFAIEDLQVEADFPVYFEELRKVLVKVDEYHSVHQKLSADMADNSNLIRSLLVRAEDARLMRDMKTMKSRYMELYDLNKDLLNGYKIRCNNHTELLGNLKAVNQAIQRAGRLRGLRWGKCHCAGDPGRHCPTSCNHRWKLCSAGFRSRRK

IRESfinder result

IRESfinder score

0.453492372

IRES/non IRES

non-IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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