top of page
mmu_circBirc6_399
Basic information of circular RNA
PanCircBase ID:
mmu_circBirc6_399
mm39_Chr. Position ID:
chr17|74936399|74938732|+
Gene name:
Birc6
Transcript:
ENSMUST00000180037.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
39,40,41
Exon/intron offsets:
0,1555,2206
Genomic length:
2333
Exon/intron sizes in nt:
117,155,127
Flanking exon/introns:
chr17:74934110-74936399|chr17:74938732-74941790
Splice length:
399
Other database IDs:
CIRCpedia: MMU_CIRCpedia_221694, circAtlas: mmu-Birc6_0042
circAtlas: hsa-BIRC6_0126
Human Ortholog IDs:
Mature spliced Sequence:
CTTTAGATGCTCGTCTAGAAGTTGGTCTTGAACAGCAAGCAGAACTTATGTTGAAAATGATGTCTACTCTGGAAGCAGACTCCATCTTACAAGCATTAACAAATACATCTCCTACCTTTTCACAATCTCCAACTGGAACAGATGACTCACTTCTGGGGAATTTACAGCCAGCAAACCAGAACAGCCAGCTCATGATACAGCTCTCATCTGTCCCAATGTTAAATGTTTGTTTCAATAAACTCTTCTCCATGCTTCAAGTCCATCATGTTCAGTTGGAGTCACTTCTTCAGTTGTGGCTCACATTGAGCCTTAATTCTAGTTCATCAGGGAACAAAGAGAATGGAGCAGATATTTTTTTATATAATGCTAATCGGATACCTGTCATCTCATTAAATCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGGGAACAAAGAGAATGGAGCA
59.62
AGATGGAGTCTGCTTCCAGA
57.76
161
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGGGAACAAAGAGAATGGAGCA
59.62
AAGATGGAGTCTGCTTCCAGA
58.46
162
PCR template sequence:
ACATTGAGCCTTAATTCTAGTTCATCAGGGAACAAAGAGAATGGAGCAGATATTTTTTTATATAATGCTAATCGGATACCTGTCATCTCATTAAATCAAGCTTTAGATGCTCGTCTAGAAGTTGGTCTTGAACAGCAAGCAGAACTTATGTTGAAAATGATGTCTACTCTGGAAGCAGACTCCATCTTACAAGCATTAAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-155-5p(1), mmu-let-7e-5p(1), mmu-miR-495-3p(1), mmu-miR-136-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TCATTAAATCAAGCTTTAGATGCTCG
siRNA sets
CTAAAGCTTGATTTAATGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBIRC6_399_ORF_1
0.8373
-0.076484091
0.801364606
750
ORF sequence:
ATGTTGAAAATGATGTCTACTCTGGAAGCAGACTCCATCTTACAAGCATTAACAAATACATCTCCTACCTTTTCACAATCTCCAACTGGAACAGATGACTCACTTCTGGGGAATTTACAGCCAGCAAACCAGAACAGCCAGCTCATGATACAGCTCTCATCTGTCCCAATGTTAAATGTTTGTTTCAATAAACTCTTCTCCATGCTTCAAGTCCATCATGTTCAGTTGGAGTCACTTCTTCAGTTGTGGCTCACATTGAGCCTTAATTCTAGTTCATCAGGGAACAAAGAGAATGGAGCAGATATTTTTTTATATAATGCTAATCGGATACCTGTCATCTCATTAAATCAAGCTTTAGATGCTCGTCTAGAAGTTGGTCTTGAACAGCAAGCAGAACTTATGTTGAAAATGATGTCTACTCTGGAAGCAGACTCCATCTTACAAGCATTAACAAATACATCTCCTACCTTTTCACAATCTCCAACTGGAACAGATGACTCACTTCTGGGGAATTTACAGCCAGCAAACCAGAACAGCCAGCTCATGATACAGCTCTCATCTGTCCCAATGTTAAATGTTTGTTTCAATAAACTCTTCTCCATGCTTCAAGTCCATCATGTTCAGTTGGAGTCACTTCTTCAGTTGTGGCTCACATTGAGCCTTAATTCTAGTTCATCAGGGAACAAAGAGAATGGAGCAGATATTTTTTTATATAATGCTAATCGGATACCTGTCATCTCATTAAATCAA
Peptide sequence:
MLKMMSTLEADSILQALTNTSPTFSQSPTGTDDSLLGNLQPANQNSQLMIQLSSVPMLNVCFNKLFSMLQVHHVQLESLLQLWLTLSLNSSSSGNKENGADIFLYNANRIPVISLNQALDARLEVGLEQQAELMLKMMSTLEADSILQALTNTSPTFSQSPTGTDDSLLGNLQPANQNSQLMIQLSSVPMLNVCFNKLFSMLQVHHVQLESLLQLWLTLSLNSSSSGNKENGADIFLYNANRIPVISLNQ
IRESfinder result
IRESfinder score
0.824913893
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page