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mmu_circBirc6_399

Basic information of circular RNA
PanCircBase ID:

mmu_circBirc6_399

mm39_Chr. Position ID:

chr17|74936399|74938732|+

Gene name:

Birc6

Transcript:

ENSMUST00000180037.8

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

3

Exon/intron indexes:

39,40,41

Exon/intron offsets:

0,1555,2206

Genomic length:

2333

Exon/intron sizes in nt:

117,155,127

Flanking exon/introns:

chr17:74934110-74936399|chr17:74938732-74941790

Splice length:

399

Other database IDs:

CIRCpedia: MMU_CIRCpedia_221694, circAtlas: mmu-Birc6_0042

circAtlas: hsa-BIRC6_0126

Human Ortholog IDs:
Mature spliced Sequence:

CTTTAGATGCTCGTCTAGAAGTTGGTCTTGAACAGCAAGCAGAACTTATGTTGAAAATGATGTCTACTCTGGAAGCAGACTCCATCTTACAAGCATTAACAAATACATCTCCTACCTTTTCACAATCTCCAACTGGAACAGATGACTCACTTCTGGGGAATTTACAGCCAGCAAACCAGAACAGCCAGCTCATGATACAGCTCTCATCTGTCCCAATGTTAAATGTTTGTTTCAATAAACTCTTCTCCATGCTTCAAGTCCATCATGTTCAGTTGGAGTCACTTCTTCAGTTGTGGCTCACATTGAGCCTTAATTCTAGTTCATCAGGGAACAAAGAGAATGGAGCAGATATTTTTTTATATAATGCTAATCGGATACCTGTCATCTCATTAAATCAAG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

AGGGAACAAAGAGAATGGAGCA

59.62

AGATGGAGTCTGCTTCCAGA

57.76

161

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

AGGGAACAAAGAGAATGGAGCA

59.62

AAGATGGAGTCTGCTTCCAGA

58.46

162

PCR template sequence:

ACATTGAGCCTTAATTCTAGTTCATCAGGGAACAAAGAGAATGGAGCAGATATTTTTTTATATAATGCTAATCGGATACCTGTCATCTCATTAAATCAAGCTTTAGATGCTCGTCTAGAAGTTGGTCTTGAACAGCAAGCAGAACTTATGTTGAAAATGATGTCTACTCTGGAAGCAGACTCCATCTTACAAGCATTAAC

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

4

Interacting miRNAs (#binding sites)

mmu-miR-155-5p(1), mmu-let-7e-5p(1), mmu-miR-495-3p(1), mmu-miR-136-5p(1)

siRNAs for circular RNA silencing
Junction sequence

TCATTAAATCAAGCTTTAGATGCTCG

siRNA sets

CTAAAGCTTGATTTAATGA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCBIRC6_399_ORF_1

0.8373

-0.076484091

0.801364606

750

ORF sequence:

ATGTTGAAAATGATGTCTACTCTGGAAGCAGACTCCATCTTACAAGCATTAACAAATACATCTCCTACCTTTTCACAATCTCCAACTGGAACAGATGACTCACTTCTGGGGAATTTACAGCCAGCAAACCAGAACAGCCAGCTCATGATACAGCTCTCATCTGTCCCAATGTTAAATGTTTGTTTCAATAAACTCTTCTCCATGCTTCAAGTCCATCATGTTCAGTTGGAGTCACTTCTTCAGTTGTGGCTCACATTGAGCCTTAATTCTAGTTCATCAGGGAACAAAGAGAATGGAGCAGATATTTTTTTATATAATGCTAATCGGATACCTGTCATCTCATTAAATCAAGCTTTAGATGCTCGTCTAGAAGTTGGTCTTGAACAGCAAGCAGAACTTATGTTGAAAATGATGTCTACTCTGGAAGCAGACTCCATCTTACAAGCATTAACAAATACATCTCCTACCTTTTCACAATCTCCAACTGGAACAGATGACTCACTTCTGGGGAATTTACAGCCAGCAAACCAGAACAGCCAGCTCATGATACAGCTCTCATCTGTCCCAATGTTAAATGTTTGTTTCAATAAACTCTTCTCCATGCTTCAAGTCCATCATGTTCAGTTGGAGTCACTTCTTCAGTTGTGGCTCACATTGAGCCTTAATTCTAGTTCATCAGGGAACAAAGAGAATGGAGCAGATATTTTTTTATATAATGCTAATCGGATACCTGTCATCTCATTAAATCAA

Peptide sequence:

MLKMMSTLEADSILQALTNTSPTFSQSPTGTDDSLLGNLQPANQNSQLMIQLSSVPMLNVCFNKLFSMLQVHHVQLESLLQLWLTLSLNSSSSGNKENGADIFLYNANRIPVISLNQALDARLEVGLEQQAELMLKMMSTLEADSILQALTNTSPTFSQSPTGTDDSLLGNLQPANQNSQLMIQLSSVPMLNVCFNKLFSMLQVHHVQLESLLQLWLTLSLNSSSSGNKENGADIFLYNANRIPVISLNQ

IRESfinder result

IRESfinder score

0.824913893

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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