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mmu_circBrinp2_345
Basic information of circular RNA
PanCircBase ID:
mmu_circBrinp2_345
mm39_Chr. Position ID:
chr1|158122995|158123340|-
Gene name:
Brinp2
Transcript:
ENSMUST00000195271.5
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
3
Exon/intron offsets:
0
Genomic length:
345
Exon/intron sizes in nt:
345
Flanking exon/introns:
chr1:158095590-158122995|chr1:158123340-158183086
Splice length:
345
Other database IDs:
CIRCpedia: MMU_CIRCpedia_10929, circAtlas: mmu-Brinp2_0002
Human Ortholog IDs:
Mature spliced Sequence:
TTTGGCGCCCTGGAGGAGCAGCGCGGAGTGGGAGAGCTTGGCGAGAGAATGAAGACACTAACCGCCTGGGAGAAGCATGAGGTGGCCGTGCAGCTCTTGGTTTAGAGGGCTTTGGCCGGAGGCCGCCCCATGGGCAGTCCTGCTGGCGCTAGGCGTACCCGGGTGGGTGCTGGCTGTCTCGGCCACGGTGGCCGCTGTGGTCCCGGAACAGCATGTCTCCTCAGCTGGCCAGGCTCCCCTGGACTGGCTGCTCACAGACCGAGGCCCCTTCCACCGAGCCCAGGAGTATGCTGATTTCATGGAGCGTTACCGCCAGGGGTTCACCACCAGATACAGGATCTACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CACCGAGCCCAGGAGTATG
59.56
CACCTCATGCTTCTCCCAGG
60.11
158
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CACCGAGCCCAGGAGTATG
59.56
CCACCTCATGCTTCTCCCAG
60.11
159
PCR template sequence:
GGCTGCTCACAGACCGAGGCCCCTTCCACCGAGCCCAGGAGTATGCTGATTTCATGGAGCGTTACCGCCAGGGGTTCACCACCAGATACAGGATCTACAGTTTGGCGCCCTGGAGGAGCAGCGCGGAGTGGGAGAGCTTGGCGAGAGAATGAAGACACTAACCGCCTGGGAGAAGCATGAGGTGGCCGTGCAGCTCTTGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
ACAGGATCTACAGTTTGGCGCCCTGG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBRINP2_345_ORF_1
0.9153
0.218309845
0.413840731
321
ORF sequence:
ATGAGGTGGCCGTGCAGCTCTTGGTTTAGAGGGCTTTGGCCGGAGGCCGCCCCATGGGCAGTCCTGCTGGCGCTAGGCGTACCCGGGTGGGTGCTGGCTGTCTCGGCCACGGTGGCCGCTGTGGTCCCGGAACAGCATGTCTCCTCAGCTGGCCAGGCTCCCCTGGACTGGCTGCTCACAGACCGAGGCCCCTTCCACCGAGCCCAGGAGTATGCTGATTTCATGGAGCGTTACCGCCAGGGGTTCACCACCAGATACAGGATCTACAGTTTGGCGCCCTGGAGGAGCAGCGCGGAGTGGGAGAGCTTGGCGAGAGAATGA
Peptide sequence:
MRWPCSSWFRGLWPEAAPWAVLLALGVPGWVLAVSATVAAVVPEQHVSSAGQAPLDWLLTDRGPFHRAQEYADFMERYRQGFTTRYRIYSLAPWRSSAEWESLARE
IRESfinder result
IRESfinder score
0.269332235
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
RPF, cORF, m6A
mmu_circBrinp2_001
Evidence
Peptide sequence:
MRWPCSSWFRGLWPEAAPWAVLLALGVPGWVLAVSATVAAVVPEQHVSSAGQAPLDWLLTDRGPFHRAQEYADFMERYRQGFTTRYRIYSLAPWRSSAEWESLARE*
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